Document Detail


Dependence of Basipetal Polar Transport of Auxin upon Aerobic Metabolism.
MedLine Citation:
PMID:  16656578     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
The movement of IAA-(14)C through coleoptile segments of Avena and Zea has been investigated under aerobic and anaerobic conditions. The results are as follows: Zea. Using a 5-mm segment and a 2-hour transport period anaerobic conditions reduced the total uptake of (14)C from an apical donor by 74% and the proportion of the total found in the receiving block by at least 45%. Anaerobic conditions reduced total uptake from a basal donor by 58% but no (14)C reached the apical receiving block in either air or N(2). Uptake from apical and basal donor blocks in N(2) is closely similar.The presence of (14)C in the basal receiving blocks, and its absence in the apical receiving blocks, in N(2) suggests that even in anaerobic conditions movement of IAA is polarized basipetally, although the movement occurs at only a fraction of the rate found in air.Anaerobic conditions induced a similar reduction in basipetal movement of IAA in upper and lower 5-mm segments taken from the apical 10 mm of a Zea coleoptile.Using 10-mm Zea segments no (14)C was recovered in the receiving blocks at the basal end of the segment after 2 and 4 hours in N(2) whereas large amounts were recovered in air.Avena: Using 5-mm segments and a 2-hour transport period the total uptake of (14)C from an apical donor is reduced by 83%. Movement of (14)C into the basal donor is totally inhibited in N(2). Total uptake of (14)C from a basal donor is reduced by 61% in nitrogen and no (14)C reached the apical receiving blocks regardless of the atmospheric conditions.A time course for the movement of (14)C into the basal and apical receiving blocks through 5-mm segments showed that in air the amount in the basal receivers increased for 4 hours and then remained approximately uniform. In N(2) no significant (14)C reached the receivers until 6 to 8 hours after the application of donors but even then the amounts were about 12 to 14% of that in aerobic receivers. Movement of (14)C into apical receivers was similar in air and in nitrogen and even after 6 to 8 hours the amount of radioactivity barely reached significant levels.
Authors:
M B Wilkins; M Martin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Plant physiology     Volume:  42     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  1967 Jun 
Date Detail:
Created Date:  2010-06-29     Completed Date:  2010-06-29     Revised Date:  2010-09-15    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  831-9     Citation Subset:  -    
Affiliation:
School of Biological Sciences, University of East Anglia, Norwich, NOR. 77H, Great Britain.
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