Document Detail

Deoxyribonucleotide biosynthesis in yeast: assay and properties of ribonucleotide reductase in permeabilized Saccharomyces cerevisiae cells.
MedLine Citation:
PMID:  3511846     Owner:  NLM     Status:  MEDLINE    
Yeast cells permeabilized by freeze-thaw cycles in a sorbitol-containing medium provide an experimentally favorable system for the study of ribonucleotide reduction in a small number of cells or in mutant strains. Ribonucleotide reductase activities determined in such cells are about twice those found in cell extracts but properties of the enzyme, except pH optimum, are closely comparable in both assay procedures. In contrast with other organisms, the activities measured in permeabilized cells from both diploid or haploid strains exceed the demand for deoxyribonucleotide formation during replication of the yeast genome. The method has been applied to yeast cultures growing in the presence of the ribonucleotide reductase inhibitor hydroxyurea and a twofold increase of enzyme activity has been established in such cells. On the other hand, analysis of a series of hus mutants, selected for hydroxyurea sensitivity in the laboratory of Singer and Johnston did not reveal obvious alterations of the enzyme vs the parental strains, suggesting that the hus phenotype may be due to lesions other than in ribonucleotide reductase.
M Lammers; H Follmann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of biochemistry and biophysics     Volume:  244     ISSN:  0003-9861     ISO Abbreviation:  Arch. Biochem. Biophys.     Publication Date:  1986 Feb 
Date Detail:
Created Date:  1986-03-13     Completed Date:  1986-03-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372430     Medline TA:  Arch Biochem Biophys     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  430-8     Citation Subset:  IM    
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MeSH Terms
Cell Membrane Permeability
DNA Replication
DNA, Fungal / analysis
Deoxyribonucleotides / biosynthesis*
Genes, Fungal
Hydroxyurea / pharmacology
Ribonucleotide Reductases / analysis*,  antagonists & inhibitors
Saccharomyces cerevisiae / drug effects,  enzymology*,  genetics
Reg. No./Substance:
0/DNA, Fungal; 0/Deoxyribonucleotides; 127-07-1/Hydroxyurea; EC 1.17.4.-/Ribonucleotide Reductases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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