Document Detail

Deoxyribonucleic acid synthesis during the division cycle of Escherichia coli: a comparison of strains B-r, K-12, 15, and 15T- under conditions of slow growth.
MedLine Citation:
PMID:  4591948     Owner:  NLM     Status:  MEDLINE    
The rates of deoxyribonucleic acid (DNA) synthesis during the division cycles of the Escherichia coli strains B/r, K-12 3000, 15T(-), and 15 have been measured in synchronous cultures, under several conditions of slow growth. These synchronous cultures were obtained by sucrose gradient centrifugation of exponentially growing cultures, after which the smallest cells were removed from the gradient and allowed to grow. Sucrose gradient centrifugation did not adversely affect the cell cycle, since an experiment in which an exponentially growing culture was pulsed with [(3)H]thymidine prior to the periodic separation and assay of the smallest cells resulted in the same conclusions, as given below. In the strains of E. coli that were studied, a decreased rate of [(3)H]thymidine incorporation was seen late in the cell cycle, prior to cell division. No decrease in the rate of [(3)H]thymidine incorporation was seen at or near the beginning of the cell cycle. Thus, all these strains appear to regulate DNA synthesis in a similar fashion during slow growth. In addition, a correlation between the appearance of cells with visible cross-walls and the start of a new round of DNA synthesis was seen, indicating that these two events might be related.
L J Gudas; A B Pardee
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  117     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1974 Mar 
Date Detail:
Created Date:  1974-04-18     Completed Date:  1974-04-18     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1216-23     Citation Subset:  IM    
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MeSH Terms
Acetates / metabolism
Aspartic Acid / metabolism
Cell Count
Cell Division*
Cell Wall
Centrifugation, Density Gradient
Culture Media
DNA Replication
DNA, Bacterial / biosynthesis*
Escherichia coli / cytology*,  growth & development
Proline / metabolism
Succinates / metabolism
Thymidine / metabolism
Thymine / metabolism
Time Factors
Reg. No./Substance:
0/Acetates; 0/Culture Media; 0/DNA, Bacterial; 0/Succinates; 10028-17-8/Tritium; 147-85-3/Proline; 50-89-5/Thymidine; 56-84-8/Aspartic Acid; 57-50-1/Sucrose; 65-71-4/Thymine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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