| Dentin matrix protein 1 initiates hydroxyapatite formation in vitro. | |
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MedLine Citation:
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PMID: 12952204 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bone and dentin formation are interesting examples of matrix-mediated mineralization. However, factors and mechanisms regulating this process are poorly understood. Dentin matrix protein 1 (DMP1) is an acidic extracellular matrix protein found in dentin and bone, and based on its amino acid composition it could be postulated to play an important role in mineralization. Our present study examines the ability of recombinant DMP1 to initiate apatite formation in vitro. A 45Ca-binding assay demonstrated that recombinant DMP1 (rDMP1) possesses calcium-binding ability under physiological conditions. The in vitro nucleation experiments when conducted with rDMP1-coated glass plates demonstrated hydroxyapatite nucleation, while amorphous mineral was deposited on blank or BSA-coated surface. This mineral deposition was found to be 10-fold higher on rDMP1-coated glass surface when compared with the control glass plates. These findings suggest that DMP1 could be considered as a nucleator for apatite deposition in vitro. |
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Authors:
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Gen He; Thomas Dahl; Arthur Veis; Anne George |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Connective tissue research Volume: 44 Suppl 1 ISSN: 0300-8207 ISO Abbreviation: Connect. Tissue Res. Publication Date: 2003 |
Date Detail:
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Created Date: 2003-09-03 Completed Date: 2004-01-05 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0365263 Medline TA: Connect Tissue Res Country: United States |
Other Details:
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Languages: eng Pagination: 240-5 Citation Subset: IM |
Affiliation:
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Department of Oral Biology, University of Illinois at Chicago, Chicago, Illinois 60612, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Calcification, Physiologic
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physiology* Calcium / metabolism Calcium Radioisotopes / diagnostic use Crystallization Escherichia coli Hydroxyapatites / chemistry, metabolism* Phosphoproteins / chemistry, metabolism*, ultrastructure Recombinant Proteins / chemistry, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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DE 11657/DE/NIDCR NIH HHS; DE01374/DE/NIDCR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Calcium Radioisotopes; 0/Hydroxyapatites; 0/Phosphoproteins; 0/Recombinant Proteins; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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