Document Detail

Dentin matrix protein 1 initiates hydroxyapatite formation in vitro.
MedLine Citation:
PMID:  12952204     Owner:  NLM     Status:  MEDLINE    
Bone and dentin formation are interesting examples of matrix-mediated mineralization. However, factors and mechanisms regulating this process are poorly understood. Dentin matrix protein 1 (DMP1) is an acidic extracellular matrix protein found in dentin and bone, and based on its amino acid composition it could be postulated to play an important role in mineralization. Our present study examines the ability of recombinant DMP1 to initiate apatite formation in vitro. A 45Ca-binding assay demonstrated that recombinant DMP1 (rDMP1) possesses calcium-binding ability under physiological conditions. The in vitro nucleation experiments when conducted with rDMP1-coated glass plates demonstrated hydroxyapatite nucleation, while amorphous mineral was deposited on blank or BSA-coated surface. This mineral deposition was found to be 10-fold higher on rDMP1-coated glass surface when compared with the control glass plates. These findings suggest that DMP1 could be considered as a nucleator for apatite deposition in vitro.
Gen He; Thomas Dahl; Arthur Veis; Anne George
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Connective tissue research     Volume:  44 Suppl 1     ISSN:  0300-8207     ISO Abbreviation:  Connect. Tissue Res.     Publication Date:  2003  
Date Detail:
Created Date:  2003-09-03     Completed Date:  2004-01-05     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0365263     Medline TA:  Connect Tissue Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  240-5     Citation Subset:  IM    
Department of Oral Biology, University of Illinois at Chicago, Chicago, Illinois 60612, USA.
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MeSH Terms
Calcification, Physiologic / physiology*
Calcium / metabolism
Calcium Radioisotopes / diagnostic use
Escherichia coli
Hydroxyapatites / chemistry,  metabolism*
Phosphoproteins / chemistry,  metabolism*,  ultrastructure
Recombinant Proteins / chemistry,  metabolism
Grant Support
Reg. No./Substance:
0/Calcium Radioisotopes; 0/Hydroxyapatites; 0/Phosphoproteins; 0/Recombinant Proteins; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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