Document Detail


Dentin matrix protein-1, a candidate gene for dentinogenesis imperfecta.
MedLine Citation:
PMID:  9084665     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dentinogenesis imperfecta (DGI) is an autosomal dominant inherited dental disease which affects dentin production and mineralization. Genetic linkage studies have determined linkage between DGI type II and group-specific component (Gc, vitamin D binding protein), interferon (gamma)-induced cytokine protein 10 (INP10) and secreted phosphoprotein 1 (SSP1, osteopontin, bone sialoprotein 1, early T-lymphocyte activation 1). Therefore, the gene locus has been localized to the long arm of human chromosome 4 in the region 4q13-q21. Dentin matrix protein-1 (DMP-1, AG-1) is a new acidic, phosphorylated dentin extracellular matrix protein which has recently been identified by cDNA cloning. The purpose of this study was to establish the possible association of DMP-1 with DGI type II by determining the human chromosomal localization of this protein. A DMP-1 DNA probe was generated1using PCR amplification of the mouse full-length DMP-1 and labeled with [32P] d-CTP. A panel of rodent somatic cell hybrid clones, previously cytogenetically characterized, was used for the assignment. High stringently DNA hybridization studies and analysis of the chromosomal cell panel indicated that the DMP-1 gene locus is located on human chromosome 4. This data supports the hypothesis that DMP-1 is a candidate gene for the genetic disease DGI type II. This is based on chromosomal localization to human chromosome 4, the expression of DMP-1 mostly by odontoblasts, and its purported physical-chemical properties.
Authors:
M MacDougall; T T Gu; D Simmons
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Connective tissue research     Volume:  35     ISSN:  0300-8207     ISO Abbreviation:  Connect. Tissue Res.     Publication Date:  1996  
Date Detail:
Created Date:  1997-05-15     Completed Date:  1997-05-15     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0365263     Medline TA:  Connect Tissue Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  267-72     Citation Subset:  IM    
Affiliation:
University of Texas Health Science Center, Department of Pediatric Dentistry, Dental School, San Antonio 78284-7888, USA. MacDougall@UTHSCSA.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Southern
Cell Line
Cricetinae
DNA / analysis
Dentin / metabolism
Dentinogenesis Imperfecta / genetics*
Extracellular Matrix Proteins
Genes
Humans
Hybrid Cells
Mice
Phosphoproteins / genetics*
Grant Support
ID/Acronym/Agency:
DE-09875/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/DMP1 protein, human; 0/Dmp1 protein, mouse; 0/Extracellular Matrix Proteins; 0/Phosphoproteins; 9007-49-2/DNA

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