| Dentin-derived BMP-2 and odontoblast differentiation. | |
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MedLine Citation:
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PMID: 20351355 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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It is known that stem cells from exfoliated deciduous teeth (SHED) can be induced to differentiate into odontoblasts. However, the nature of dentin-derived morphogenic signals required for dental pulp stem cell differentiation remains unclear. The hypothesis underlying this work is that dentin-derived Bone Morphogenetic Proteins (BMP) are necessary for the differentiation of SHED into odontoblasts. We observed that SHED express markers of odontoblastic differentiation (DSPP, DMP-1, MEPE) when seeded in human tooth slice/scaffolds and cultured in vitro, or implanted subcutaneously into immunodeficient mice. In contrast, SHED cultured in deproteinized tooth slice/scaffolds, or scaffolds without a tooth slice, do not express these markers. SHED express the BMP receptors BMPR-IA, BMPR-IB, and BMPR-II. Notably, blockade of BMP-2 signaling inhibited the expression of markers of odontoblastic differentiation by SHED cultured in tooth slice/scaffolds. Collectively, this work demonstrates that dentin-derived BMP-2 is required to induce the differentiation of SHED into odontoblasts. |
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Authors:
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L Casagrande; F F Demarco; Z Zhang; F B Araujo; S Shi; J E N?r |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-29 |
Journal Detail:
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Title: Journal of dental research Volume: 89 ISSN: 1544-0591 ISO Abbreviation: J. Dent. Res. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-05-17 Completed Date: 2010-06-11 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0354343 Medline TA: J Dent Res Country: United States |
Other Details:
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Languages: eng Pagination: 603-8 Citation Subset: D; IM |
Affiliation:
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Department of Pediatric Dentistry, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, Brazil. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Neutralizing / pharmacology Biological Markers / analysis Blotting, Western Bone Morphogenetic Protein 2 / antagonists & inhibitors, pharmacology* Bone Morphogenetic Protein 7 / antagonists & inhibitors, pharmacology Bone Morphogenetic Protein Receptors, Type I / analysis Bone Morphogenetic Protein Receptors, Type II / analysis Cell Differentiation / drug effects, physiology Cells, Cultured Dental Pulp / cytology Dentin / enzymology* Extracellular Matrix Proteins / analysis Glycoproteins / analysis Humans Mice Mice, SCID Odontoblasts / drug effects* Phosphoproteins / analysis Reverse Transcriptase Polymerase Chain Reaction Sialoglycoproteins / analysis Signal Transduction / drug effects Stem Cells / drug effects, physiology Subcutaneous Tissue / surgery Tissue Scaffolds Tooth, Deciduous / cytology |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Neutralizing; 0/BMP2 protein, human; 0/BMP7 protein, human; 0/Biological Markers; 0/Bone Morphogenetic Protein 2; 0/Bone Morphogenetic Protein 7; 0/DMP1 protein, human; 0/Extracellular Matrix Proteins; 0/Glycoproteins; 0/MEPE protein, human; 0/Phosphoproteins; 0/Sialoglycoproteins; 0/dentin sialophosphoprotein; EC 2.7.1.37/BMPR1A protein, human; EC 2.7.1.37/BMPR1B protein, human; EC 2.7.1.37/BMPR2 protein, human; EC 2.7.11.30/Bone Morphogenetic Protein Receptors, Type I; EC 2.7.11.30/Bone Morphogenetic Protein Receptors, Type II |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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