Document Detail


Density gradient separation of sperm for artificial insemination.
MedLine Citation:
PMID:  22992916     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Human spermatozoa for clinical procedures such as IUI or IVF, or for diagnostic or research studies of sperm fertilizing ability, must be separated from the seminal plasma environment not only as soon as possible after ejaculation but also as efficiently as possible, minimizing seminal plasma and bacterial carryover. Furthermore, in addition to technical simplicity and robustness, a sperm preparation method needs to select not just the more motile and morphologically normal spermatozoa but also those spermatozoa with reduced DNA damage. Currently the most effective and efficient technique for this is density gradient centrifugation, which has been extensively validated through research and clinical application. An optimized protocol based on silane-coated colloidal silica products is provided.
Authors:
David Mortimer; Sharon T Mortimer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  927     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-09-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  217-26     Citation Subset:  IM    
Affiliation:
Oozoa Biomedical Inc., West Vancouver, BC, Canada, david@oozoa.com.
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