| Density gradient separation of sperm for artificial insemination. | |
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MedLine Citation:
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PMID: 22992916 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Human spermatozoa for clinical procedures such as IUI or IVF, or for diagnostic or research studies of sperm fertilizing ability, must be separated from the seminal plasma environment not only as soon as possible after ejaculation but also as efficiently as possible, minimizing seminal plasma and bacterial carryover. Furthermore, in addition to technical simplicity and robustness, a sperm preparation method needs to select not just the more motile and morphologically normal spermatozoa but also those spermatozoa with reduced DNA damage. Currently the most effective and efficient technique for this is density gradient centrifugation, which has been extensively validated through research and clinical application. An optimized protocol based on silane-coated colloidal silica products is provided. |
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Authors:
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David Mortimer; Sharon T Mortimer |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 927 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2013 |
Date Detail:
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Created Date: 2012-09-20 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 217-26 Citation Subset: IM |
Affiliation:
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Oozoa Biomedical Inc., West Vancouver, BC, Canada, david@oozoa.com. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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