Document Detail

Density-dependent regulation of epidermal growth factor receptor expression.
MedLine Citation:
PMID:  1716909     Owner:  NLM     Status:  MEDLINE    
Previous studies have demonstrated that binding of peptide growth factors such as epidermal growth factor (EGF) decreases as cell density increases. We now report that binding of EGF to MDA 468 breast carcinoma cells is reduced as cells increase in density with time in culture. Cells at low density bound more EGF per cell than cells at higher density. The reduction of EGF binding was due to a reduction in receptor number. Metabolic labeling of MDA 468 cells with [35S]-methionine followed by immunoprecipitation of the EGF receptor (EGFR) indicated that the amount of total receptor protein was decreased. Using RNA blot hybridization, we found that high-density cells contained decreased amounts of EGFR transcripts. Northern analysis revealed that both the 10- and 5.6-kilobase mRNA transcripts encoding the EGFR were decreased. These data suggest that increasing cell density with time in culture results in modulation of EGFR content, with changes at the level of both protein and mRNA expression.
A W Hamburger; D Mehta; G Pinnamaneni; L C Chen; Y Reid
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Pathobiology : journal of immunopathology, molecular and cellular biology     Volume:  59     ISSN:  1015-2008     ISO Abbreviation:  Pathobiology     Publication Date:  1991  
Date Detail:
Created Date:  1991-10-30     Completed Date:  1991-10-30     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9007504     Medline TA:  Pathobiology     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  329-34     Citation Subset:  IM    
University of Maryland Cancer Center, Baltimore.
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MeSH Terms
Blotting, Northern
Breast Neoplasms / metabolism,  pathology*,  ultrastructure
Cell Count
Down-Regulation / physiology
Epidermal Growth Factor / metabolism
Gene Expression Regulation, Neoplastic / physiology*
Methionine / metabolism
Nucleic Acid Hybridization
Precipitin Tests
RNA / genetics,  metabolism
Receptor, Epidermal Growth Factor / genetics,  metabolism*
Sulfur Radioisotopes / diagnostic use
Transcription, Genetic
Tumor Cells, Cultured / metabolism,  pathology,  ultrastructure
Grant Support
Reg. No./Substance:
0/Sulfur Radioisotopes; 62229-50-9/Epidermal Growth Factor; 63-68-3/Methionine; 63231-63-0/RNA; EC, Epidermal Growth Factor

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