Document Detail


Densin is a novel cell membrane protein of Sertoli cells in the testis.
MedLine Citation:
PMID:  17039495     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell-cell interactions between Sertoli cells and germ cells are crucial for the maturation of germ cells in spermatogenesis but the structural and functional aspects of the interactions remain to be fully elucidated. Densin is a junction protein suggested to play a role in establishment of specific cell-cell contacts in the post-synaptic densities of the brain and the slit diaphragm of the kidney podocyte. In the present study, densin was discovered to be expressed in the testis of the man and the mouse. Expression of densin at the gene and the protein level was studied by using RT-PCR and Western blotting analyses, and the localization of densin was explored with immunofluorescence staining. RT-PCR and Western blotting analyses showed that densin is expressed at the gene and the protein levels. Immunofluorescence staining localized the expression of densin to the cell membranes of Sertoli cells suggesting that densin may be an adherens junction protein between Sertoli cells and developing germ cells. Densin is a novel testicular protein expressed in the cell membranes of Sertoli cells. Its functional role remains to be assessed.
Authors:
Markus Lassila; Juuso Juhila; Eija Heikkilä; Harry Holthöfer
Related Documents :
8496325 - Is infancy a quiescent period of testicular development? histological, morphometric, an...
6802195 - Immunocytochemical evidence for the specific localization of aldose reductase in rat se...
2434375 - Morphogenetic restructuring and formation of basement membranes by sertoli cells and te...
7673875 - Promotion of differentiation of newt primary spermatocytes into spermatids by mammalian...
19378385 - Evidence of multistability in a realistic computer simulation of hippocampus subfield ca1.
23070725 - Curcumin disturbed cell-cycle distribution of hepg2 cells via cytoskeletal arrangement.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular reproduction and development     Volume:  74     ISSN:  1040-452X     ISO Abbreviation:  Mol. Reprod. Dev.     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-03-05     Completed Date:  2007-08-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8903333     Medline TA:  Mol Reprod Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  641-5     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2006 Wiley-Liss, Inc.
Affiliation:
Research Program in Molecular Medicine, Biomedicum, University of Helsinki, Helsinki, Finland. markus.lassila@helsinki.fi
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adult
Animals
Cadherins / metabolism
Cell Membrane / metabolism
Humans
Male
Membrane Proteins / metabolism*
Mice
Phosphoproteins / metabolism
Sertoli Cells / metabolism*
Sialoglycoproteins / metabolism*
Testis / metabolism
beta Catenin / metabolism
Chemical
Reg. No./Substance:
0/Cadherins; 0/LRRC7 protein, human; 0/Lrrc7 protein, mouse; 0/Membrane Proteins; 0/Phosphoproteins; 0/Sialoglycoproteins; 0/beta Catenin; 0/zonula occludens-1 protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Biowaiver monographs for immediate release solid oral dosage forms: prednisolone.
Next Document:  Cancer-specific mortality after radiation therapy with short-course hormonal therapy or radical pros...