| Dengue virus type 2 NS3 protease and NS2B-NS3 protease precursor induce apoptosis. | |
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MedLine Citation:
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PMID: 12867651 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Apoptosis was detected in Vero cell cultures expressing transfected dengue virus type 2 (DENV-2) genes. Approximately 17.5 and 51.5 % of cells expressing NS3 serine protease and NS2B-NS3(185) serine protease precursor protein [NS2B-NS3(185)(pro)] genes, respectively, were apoptotic. The percentage of apoptotic cells was significantly higher in cell cultures expressing NS2B-NS3(185)(pro). NS2B-NS3(185)(pro) was detected as NS2B-NS3(185)(pro)-EGFP fusion protein in cytoplasmic vesicular structures in the apoptotic cells. Site-directed mutagenesis which replaced His(51) with Ala within the protease catalytic triad significantly reduced the ability of the expressed NS3 and NS2B-NS3(185)(pro) to induce apoptosis. Results from the present study showed that DENV-2-encoded NS3 serine protease induces apoptosis, which is enhanced in cells expressing its precursor, NS2B-NS3(185)(pro). These findings suggest the importance of NS2B as a cofactor to NS3 protease-induced apoptosis. |
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Authors:
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Norazizah Shafee; Sazaly AbuBakar |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of general virology Volume: 84 ISSN: 0022-1317 ISO Abbreviation: J. Gen. Virol. Publication Date: 2003 Aug |
Date Detail:
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Created Date: 2003-07-17 Completed Date: 2003-08-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0077340 Medline TA: J Gen Virol Country: England |
Other Details:
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Languages: eng Pagination: 2191-5 Citation Subset: IM |
Affiliation:
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Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Apoptosis* Cercopithecus aethiops Dengue Virus / pathogenicity* Green Fluorescent Proteins Humans Luminescent Proteins / genetics, metabolism Mice Molecular Sequence Data Protein Precursors / metabolism* RNA Helicases Recombinant Fusion Proteins / genetics, metabolism Serine Endopeptidases Vero Cells Viral Nonstructural Proteins / chemistry, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Luminescent Proteins; 0/NS2B protein, flavivirus; 0/NS3 protein, flavivirus; 0/Protein Precursors; 0/Recombinant Fusion Proteins; 0/Viral Nonstructural Proteins; 147336-22-9/Green Fluorescent Proteins; EC 2.7.7.-/RNA Helicases; EC 3.4.21.-/Serine Endopeptidases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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