Document Detail


Dendroflorin retards the senescence of MRC-5 cells.
MedLine Citation:
PMID:  18468396     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The influence of dendroflorin, a potentially active compound extracted from Dendrobium nobile, on cell growth and cell cycles was assessed in a human embryo lung fibroblastic MRC-5 cell line. An MTT assay indicated that dendroflorin benefits cell proliferation. Additionally, we used flow cytometry to examine the cell cycles in senescent MRC-5 cells after treatment with dendroflorin, discovering that dendroflorin could trigger cells in the G1 phase to enter the S phase. Further examination of intracellular ROS contents using DCFH-DA suggested that dendroflorin helps ROS degradation, partly elucidating the mechanism of its effects. All of the above results suggest that dendroflorin is a potential candidate with anti-senescence activity.
Authors:
Jinghua Jin; Yu Liang; Hong Xie; Xue Zhang; Xinsheng Yao; Zhao Wang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Die Pharmazie     Volume:  63     ISSN:  0031-7144     ISO Abbreviation:  Pharmazie     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-05-12     Completed Date:  2008-06-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9800766     Medline TA:  Pharmazie     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  321-3     Citation Subset:  IM    
Affiliation:
Protein Science Key Laboratory of the Ministry of Education, Department of Biological Sciences and Biotechnology, School of Medicine, Tsinghua University, Beijing, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Cell Aging / drug effects*
Cell Cycle / drug effects
Cell Line
Dendrobium / chemistry
Flow Cytometry
Free Radical Scavengers / pharmacology*
G1 Phase / drug effects
Humans
Oxidative Stress / drug effects
Reactive Oxygen Species / metabolism
S Phase / drug effects
Tetrazolium Salts
Thiazoles
Chemical
Reg. No./Substance:
0/Free Radical Scavengers; 0/Reactive Oxygen Species; 0/Tetrazolium Salts; 0/Thiazoles; 298-93-1/thiazolyl blue

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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