Document Detail


Dendritic cells in acute promyelocytic leukaemia.
MedLine Citation:
PMID:  11564070     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dendritic cell (DC) differentiation was investigated in samples from two acute promyelocytic leukaemia (APL) patients with classic translocation t(15;17)(q22;q21). After 18 d of culture in the presence of granulocyte-macrophage colony-stimulating factor, interleukin 4 and tumour necrosis factor alpha, 10-15% of pathological promyelocytes had differentiated into DC-like cells, as demonstrated by immunological and functional characteristics and by analysis of CD1a+ cells. In one patient, analysed at relapse and after developing a picture of secondary myelodysplastic syndrome (MDS), three different populations of DCs were demonstrated, two of which derived from pathological myeloid precursors (the APL and the MDS clones). This patient's DCs also presented abnormal dextran uptake. Our results demonstrated that pathological myeloid precursors in APL can differentiate into DC-like elements and that different populations of pathological DCs may coexist in the same patient.
Authors:
G M Rigolin; M Della Porta; R Bigoni; A Tieghi; A Cuneo; G Castoldi
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  British journal of haematology     Volume:  114     ISSN:  0007-1048     ISO Abbreviation:  Br. J. Haematol.     Publication Date:  2001 Sep 
Date Detail:
Created Date:  2001-09-20     Completed Date:  2001-12-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372544     Medline TA:  Br J Haematol     Country:  England    
Other Details:
Languages:  eng     Pagination:  830-3     Citation Subset:  IM    
Affiliation:
Section of Haematology, Department of Biomedical Sciences, University of Ferrara, Corso Giovecca, 203, 44100 Ferrara, Italy. sse@dne.unife.it
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD1 / analysis
Cell Differentiation
Cells, Cultured
Chromosomes, Human, Pair 15
Chromosomes, Human, Pair 17
Dendritic Cells / immunology*,  metabolism
Dextrans / metabolism
Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology
Humans
Immunophenotyping
In Situ Hybridization, Fluorescence
Interleukin-4 / pharmacology
Leukemia, Promyelocytic, Acute / genetics,  immunology*,  metabolism
Myelodysplastic Syndromes / immunology
Translocation, Genetic
Tumor Necrosis Factor-alpha / pharmacology
Chemical
Reg. No./Substance:
0/Antigens, CD1; 0/Tumor Necrosis Factor-alpha; 207137-56-2/Interleukin-4; 83869-56-1/Granulocyte-Macrophage Colony-Stimulating Factor; 9004-54-0/Dextrans

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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