Document Detail


Dendritic cells enhance the differentiation of naïve B cells into plasma cells in vitro.
MedLine Citation:
PMID:  9874489     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have shown previously that in vitro-generated human dendritic cells have an effect on the response of B cells at various stages of their differentiation. In a culture system described for the in vitro induction of plasma-cell differentiation, it was reported that naïve B cells have a poor propensity to differentiate into plasma cells. In such a culture system, 12% of naïve B cells differentiated into plasma cells in the presence of IL-2 and IL-10, despite the interruption of CD40 signalling which is necessary for plasma-cell differentiation. However, as reported herein, naïve B cells differentiated fully into plasma cells in response to dendritic cells. Addition of dendritic cells enhanced this differentiation strikingly by recruiting 57% of B cells as plasma cells producing IgM, but also IgG and IgA. In this model, dendritic cells act in synergy with IL-2 at an early stage of CD40-dependent B-cell differentiation, while IL-2 and IL-10 act together, at a later stage, in the generation of plasma cells in a CD40-independent manner. Thus, in addition to the key role played by dendritic cells in the initiation of T-cell responses, our results suggest that dendritic cells regulate humoral responses.
Authors:
J Fayette; I Durand; J M Bridon; C Arpin; B Dubois; C Caux; Y J Liu; J Banchereau; F Brière
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Scandinavian journal of immunology     Volume:  48     ISSN:  0300-9475     ISO Abbreviation:  Scand. J. Immunol.     Publication Date:  1998 Dec 
Date Detail:
Created Date:  1999-01-22     Completed Date:  1999-01-22     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0323767     Medline TA:  Scand J Immunol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  563-70     Citation Subset:  IM    
Affiliation:
Schering-Plough, Laboratory for Immunological Research, Dardilly, France.
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD40 / metabolism
B-Lymphocytes / cytology*,  drug effects,  metabolism
Cell Differentiation
Dendritic Cells / metabolism*
Humans
Immunoglobulin D / metabolism
Immunoglobulins / biosynthesis
Interleukin-10 / metabolism,  pharmacology
Interleukin-12 / metabolism
Interleukin-2 / pharmacology
Interleukin-6 / metabolism
Lymphocyte Activation
Plasma Cells / cytology*
Chemical
Reg. No./Substance:
0/Antigens, CD40; 0/Immunoglobulin D; 0/Immunoglobulins; 0/Interleukin-2; 0/Interleukin-6; 130068-27-8/Interleukin-10; 187348-17-0/Interleukin-12

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