| Dendritic cells adhere to and transmigrate across lymphatic endothelium in response to IFN-α. | |
| | |
MedLine Citation:
|
PMID: 21061437 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Migration of DC into lymphatic vessels ferries antigenic cargo and pro-inflammatory stimuli into the draining LN. Given that tissues under the influence of viral infections produce type I IFN, it is conceivable that these cytokines enhance DC migration in order to facilitate an antiviral immune response. Cultured lymphatic endothelium monolayers pretreated with TNF-α were used to model this phenomenon under inflammatory conditions. DC differentiated in the presence of either IFN-α2b or IFN-α5 showed enhanced adhesion to cultured lymphatic endothelial cells. These pro-adhesive effects were mediated by DC, not the lymphatic endothelium, and correlated with increased DC transmigration across lymphatic endothelial cell monolayers. Transmigration was guided by chemokines acting on DC, and blocking experiments with mAb indicated a role for LFA-1. Furthermore, incubation of DC with IFN-α led to the appearance of active conformation epitopes on the CD11a integrin chains expressed by DC. Differentiation of mouse DC in the presence of IFN-α also increased DC migration from inflammed footpads toward popliteal LN. Collectively, these results indicate a role for type I IFN in directing DC toward LN under inflammatory conditions. |
| | |
Authors:
|
Ana Rouzaut; Saray Garasa; Alvaro Teijeira; Iranzu González; Ivan Martinez-Forero; Natalia Suarez; Esther Larrea; Carlos Alfaro; Asís Palazón; Juan Dubrot; Sandra Hervás-Stubbs; Ignacio Melero |
Related Documents
:
|
18582937 - The porcine dendritic cell family. 16212897 - Development of dendritic cell system. 16856207 - Ifn-alpha-conditioned dendritic cells are highly efficient in inducing cross-priming cd... 16849477 - Dendritic cells transduced with socs-3 exhibit a tolerogenic/dc2 phenotype that directs... 19038457 - Dendritic cells transduced with lentiviral-mediated relb-specific shrnas inhibit the de... 11025197 - Oxidative stress-induced apoptosis of endothelial cells. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-10-27 |
Journal Detail:
|
Title: European journal of immunology Volume: 40 ISSN: 1521-4141 ISO Abbreviation: Eur. J. Immunol. Publication Date: 2010 Nov |
Date Detail:
|
Created Date: 2010-11-09 Completed Date: 2010-12-17 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 1273201 Medline TA: Eur J Immunol Country: Germany |
Other Details:
|
Languages: eng Pagination: 3054-63 Citation Subset: IM |
Copyright Information:
|
Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Affiliation:
|
Center for Applied Medical Research, School of Medicine, University of Navarra, Pamplona, Spain. arouzaut@unav.es |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antigens, CD11a / immunology Cell Adhesion / drug effects, immunology Cell Differentiation / drug effects, immunology Cell Movement / drug effects, immunology* Coculture Techniques Dendritic Cells / immunology* Endothelium, Lymphatic / immunology* Humans Interferon-alpha / immunology*, pharmacology Lymphocyte Function-Associated Antigen-1 / immunology Mice Tumor Necrosis Factor-alpha / immunology, pharmacology |
| Chemical | |
Reg. No./Substance:
|
0/Antigens, CD11a; 0/Interferon-alpha; 0/Lymphocyte Function-Associated Antigen-1; 0/Tumor Necrosis Factor-alpha; 0/interferon alpha5; 0/interferon-alpha 2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Apoptosis regulators Fas and Bim synergistically control T-lymphocyte homeostatic proliferation.
Next Document: The L1 major capsid protein of HPV16 differentially modulates APC trafficking according to the vacci...