| Dendritic cells (DCs) transfected with a recombinant adenovirus carrying chlamydial major outer membrane protein antigen elicit protective immune responses against genital tract challenge infection. | |
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MedLine Citation:
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PMID: 20651849 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Chlamydia trachomatis, an obligate intracellular bacterial pathogen, is the major cause of sexually transmitted diseases worldwide. Although a variety of strategies have been taken to promote the development of a protective vaccine, no ideal vaccine has been generated so far. In this study, we transfected dendritic cells (DCs) with recombinant adenovirus carrying C. trachomatis serovar E major outer membrane protein gene (Ad-MOMP), and investigated their ability to induce specific protection against genital tract chlamydial challenge infection. The results showed that when DCs were transfected with Ad-MOMP in vitro, the DCs exhibited increased expression of CD80 and MHC-II molecules as well as enhanced IL-12 secretion and were able to stimulate T-cell proliferation. The level of IFN-gamma secreted by stimulated T cells was also up-regulated significantly. When the Ad-MOMP transfected DCs were adoptively transferred intravenously to naive mice, they generated Th1-biased cytokine production and mucosal IgA responses specific for C. trachomatis. More importantly, the mice immunized with Ad-MOMP-DC mounted protection against genital tract challenge infection, shown by lower body mass loss, lower chlamydial loads, and less severe pathological changes. In conclusion, Ad-MOMP transfected DCs are capable of inducing effective protective immune responses against C. trachomatis genital infection. |
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Authors:
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Hui Lü; Hong Wang; Hong-Mei Zhao; Lei Zhao; Qiang Chen; Mei Qi; Juan Liu; Han Yu; Xiu-Ping Yu; Xi Yang; Wei-Ming Zhao |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemistry and cell biology = Biochimie et biologie cellulaire Volume: 88 ISSN: 1208-6002 ISO Abbreviation: Biochem. Cell Biol. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-23 Completed Date: 2010-12-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8606068 Medline TA: Biochem Cell Biol Country: Canada |
Other Details:
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Languages: eng Pagination: 757-65 Citation Subset: IM |
Affiliation:
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Department of Medical Microbiology, Shandong Univeristy School of Medicine, Jinan, Shandong, P.R. China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenoviridae
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genetics* Animals Cells, Cultured Chlamydia Infections / genetics, immunology, therapy* Chlamydia trachomatis / immunology, physiology Dendritic Cells / immunology, metabolism, physiology*, transplantation Female Genital Diseases, Female / genetics, immunology, therapy* Immunity, Innate / genetics, physiology Immunotherapy, Adoptive / methods* Mice Mice, Inbred BALB C Porins / genetics*, immunology Transfection Transgenes |
| Chemical | |
Reg. No./Substance:
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0/Porins; 146409-23-6/omp1 protein, Chlamydia trachomatis |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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