Document Detail

Dendritic cell activation, phagocytosis,and CD69 expression on cognate T cells are suppressed by n-3 long chain polyunsaturated fatty acids.
MedLine Citation:
PMID:  23373457     Owner:  NLM     Status:  Publisher    
Docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) are bioactive n-3 long chain polyunsaturated fatty acids (LCPUFAs) in fish oil that exert immunosuppressive effects. A significant amount of literature show n-3 LCPUFAssuppress dendritic cell (DC) function in vitro; however, few studies have determined if theeffects are emulatedat the animal level. In this study, we first focused on the functional consequences of 5% (wt/wt) fish oil on splenic CD11c(+) DCs. Administration of n-3 LCPUFAs, modeling human pharmacological intake (2% of total kcal from EPA,1.3% from DHA), to C57BL/6 mice for 3 weeks reduced DC surface expression of CD80 by 14% and TNFα secretion by 29% upon lipopolysaccharide stimulation relative to a control diet. N-3 LCPUFAs also significantly decreased CD11c(+) surface expression and phagocytosisby 12% compared to the control diet. Antigen presentation studies revealed a 22% decrease in CD69 surface expression on transgenic CD4(+) T lymphocytes activated by DCs from fish oil fed mice. We then determined if the functional changes were mechanistically associated with changes in lipid microdomain clustering or plasma membrane microviscosity with n-3 LCPUFAs, as reported for B and T lymphocytes.Fish oil administration to mice did not influencecholera-toxin induced lipid microdomain clustering or microviscosity,even though EPA and DHA levels were significantly elevated relative to the control diet.Overall, our data show n-3 LCPUFAs exertimmunosuppressive effects on DCs,validatingin vitro studies. The results also demonstrate DCmicrodomain clustering and microviscosity were not changed by the n-3 LCPUFA intervention used in this study. © 2013 The Authors. Immunology © 2013 Blackwell Publishing Ltd.
Heather Teague; Benjamin Drew Rockett; Mitchel Harris; David A Brown; Saameraza Shaikh
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-2-4
Journal Detail:
Title:  Immunology     Volume:  -     ISSN:  1365-2567     ISO Abbreviation:  Immunology     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-2-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374672     Medline TA:  Immunology     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2013 The Authors. Immunology © 2013 Blackwell Publishing Ltd.
Department of Biochemistry and Molecular Biology, East Carolina University; East Carolina Diabetes and Obesity Institute, East Carolina University.
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