Document Detail


Dendritic cell activation, phagocytosis and CD69 expression on cognate T cells are suppressed by n-3 long-chain polyunsaturated fatty acids.
MedLine Citation:
PMID:  23373457     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) are bioactive n-3 long-chain polyunsaturated fatty acids (LCPUFAs) in fish oil that exert immunosuppressive effects. A significant amount of literature shows that n-3 LCPUFAs suppress dendritic cell (DC) function in vitro; however, few studies have determined if the effects are emulated at the animal level. In this study, we first focused on the functional consequences of 5% (weight/weight) fish oil on splenic CD11c(+) DCs. Administration of n-3 LCPUFAs, modelling human pharmacological intake (2% of total kcal from EPA,1·3% from DHA), to C57BL/6 mice for 3 weeks reduced DC surface expression of CD80 by 14% and tumour necrosis factor-α secretion by 29% upon lipopolysaccharide stimulation relative to a control diet. The n-3 LCPUFAs also significantly decreased CD11c(+) surface expression and phagocytosis by 12% compared with the control diet. Antigen presentation studies revealed a 22% decrease in CD69 surface expression on transgenic CD4(+) T lymphocytes activated by DCs from mice fed fish oil. We then determined if the functional changes were mechanistically associated with changes in lipid microdomain clustering or plasma membrane microviscosity with n-3 LCPUFAs, as reported for B and T lymphocytes. Fish oil administration to mice did not influence cholera-toxin induced lipid microdomain clustering or microviscosity, even though EPA and DHA levels were significantly elevated relative to the control diet. Overall, our data show that n-3 LCPUFAs exert immunosuppressive effects on DCs, validating in vitro studies. The results also show that DC microdomain clustering and microviscosity were not changed by the n-3 LCPUFA intervention used in this study.
Authors:
Heather Teague; Benjamin Drew Rockett; Mitchel Harris; David A Brown; Saame Raza Shaikh
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Immunology     Volume:  139     ISSN:  1365-2567     ISO Abbreviation:  Immunology     Publication Date:  2013 Jul 
Date Detail:
Created Date:  2013-06-14     Completed Date:  2013-08-19     Revised Date:  2014-07-01    
Medline Journal Info:
Nlm Unique ID:  0374672     Medline TA:  Immunology     Country:  England    
Other Details:
Languages:  eng     Pagination:  386-94     Citation Subset:  IM    
Copyright Information:
© 2013 John Wiley & Sons Ltd.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigen Presentation
Antigens, CD / genetics,  metabolism*
Antigens, Differentiation, T-Lymphocyte / genetics,  metabolism*
Dendritic Cells / drug effects,  immunology*,  metabolism
Dietary Fats, Unsaturated / administration & dosage,  immunology,  pharmacology*
Docosahexaenoic Acids / administration & dosage,  immunology,  pharmacology
Eicosapentaenoic Acid / administration & dosage,  immunology,  pharmacology
Fatty Acids, Omega-3 / administration & dosage,  immunology,  pharmacology*
Fish Oils / administration & dosage,  immunology,  pharmacology
Humans
Lectins, C-Type / genetics,  metabolism*
Male
Mice
Mice, Inbred C57BL
Phagocytosis
T-Lymphocytes / immunology*
Grant Support
ID/Acronym/Agency:
R15AT006122/AT/NCCAM NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, CD; 0/Antigens, Differentiation, T-Lymphocyte; 0/CD69 antigen; 0/Dietary Fats, Unsaturated; 0/Fatty Acids, Omega-3; 0/Fish Oils; 0/Lectins, C-Type; 25167-62-8/Docosahexaenoic Acids; AAN7QOV9EA/Eicosapentaenoic Acid
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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