Document Detail


Dendritic cell responses to surface properties of clinical titanium surfaces.
MedLine Citation:
PMID:  20977948     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dendritic cells (DCs) play pivotal roles in responding to foreign entities during the innate immune response and in initiating effective adaptive immunity as well as maintaining immune tolerance. The sensitivity of DCs to foreign stimuli also makes them useful cells to assess the inflammatory response to biomaterials. Elucidating material property-DC phenotype relationships using a well-defined biomaterial system is expected to provide criteria for immunomodulatory biomaterial design. Clinical titanium (Ti) substrates, including pretreatment (PT), sand blasted and acid etched (SLA), and modified SLA (modSLA), with different roughnesses and surface energies were used to treat DCs and resulted in differential DC responses. PT and SLA induced a mature DC (mDC) phenotype, while modSLA promoted a non-inflammatory environment by supporting an immature DC (iDC) phenotype, based on surface marker expression, cytokine production profiles and cell morphology. Principal component analysis (PCA) confirmed these experimental results, and also indicated that the non-stimulating property of modSLA covaried with certain surface properties, such as high surface hydrophilicity, percent oxygen and percent Ti of the substrates. In addition to previous research that demonstrated superior osteogenic properties of modSLA compared with PT and SLA, the results reported herein indicates that modSLA may further benefit implant osteointegration by reducing local inflammation and its associated osteoclastogenesis.
Authors:
Peng Meng Kou; Zvi Schwartz; Barbara D Boyan; Julia E Babensee
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-10-25
Journal Detail:
Title:  Acta biomaterialia     Volume:  7     ISSN:  1878-7568     ISO Abbreviation:  Acta Biomater     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-01-31     Completed Date:  2011-05-19     Revised Date:  2012-03-07    
Medline Journal Info:
Nlm Unique ID:  101233144     Medline TA:  Acta Biomater     Country:  England    
Other Details:
Languages:  eng     Pagination:  1354-63     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
Affiliation:
Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332, USA.
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MeSH Terms
Descriptor/Qualifier:
Cell Differentiation
Cytokines / metabolism
Dendritic Cells / immunology*
Humans
Microscopy, Electron, Scanning
Osteoblasts / cytology,  metabolism,  ultrastructure
Principal Component Analysis
Surface Properties
Titanium / chemistry*
Grant Support
ID/Acronym/Agency:
AR052102/AR/NIAMS NIH HHS; EB004633/EB/NIBIB NIH HHS; R01 AR052102-04/AR/NIAMS NIH HHS; R01 EB004633-04/EB/NIBIB NIH HHS
Chemical
Reg. No./Substance:
0/Cytokines; 7440-32-6/Titanium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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