| Denaturation of nucleic acids induced by intercalating agents. Biochemical and biophysical properties of acridine orange-DNA complexes. | |
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MedLine Citation:
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PMID: 6400830 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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At high binding densities acridine orange (AO) forms complexes with ds DNA which are insoluble in aqueous media. These complexes are characterized by high red- and minimal green-luminescence, 1:1 (dye/P) stoichiometry and resemble complexes of AO with ss nucleic acids. Formation of these complexes can be conveniently monitored by light scatter measurements. Light scattering properties of these complexes are believed to result from the condensation of nucleic acids induced by the cationic, intercalating ligands. The spectral and thermodynamic data provide evidence that AO (and other intercalating agents) induces denaturation of ds nucleic acids; the driving force of the denaturation is high affinity and cooperativity of binding of these ligands to ss nucleic acids. The denaturing effects of AO, adriamycin and ellipticine were confirmed by biochemical studies on accessibility of DNA bases (in complexes with these ligands) to the external probes. The denaturing properties of AO vary depending on the primary structure (sugar- and base-composition) of nucleic acids. |
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Authors:
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J Kapuscinski; Z Darzynkiewicz |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of biomolecular structure & dynamics Volume: 1 ISSN: 0739-1102 ISO Abbreviation: J. Biomol. Struct. Dyn. Publication Date: 1984 Jun |
Date Detail:
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Created Date: 1990-03-14 Completed Date: 1990-03-14 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8404176 Medline TA: J Biomol Struct Dyn Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1485-99 Citation Subset: IM |
Affiliation:
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Memorial Sloan-Kettering Cancer Center, Walker Laboratory, Rye, New York 10580. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acridine Orange* Binding Sites DNA* Intercalating Agents Light Nucleic Acid Denaturation Scattering, Radiation |
| Grant Support | |
ID/Acronym/Agency:
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CA23296/CA/NCI NIH HHS; CA28704/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Intercalating Agents; 65-61-2/Acridine Orange; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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