Document Detail

Denaturation behaviour of DNA-protein-complexes detected in situ in metaphase chromosomes in suspension by Hoechst 33258 fluorescence.
MedLine Citation:
PMID:  1707689     Owner:  NLM     Status:  MEDLINE    
The denaturation behaviour of DNA-protein complexes in metaphase chromosomes in suspension was analysed in situ by Hoechst 33258 fluorescence. The results indicate that due to the stability of the dye molecule and the product of the molecular extinction coefficient and the quantum yield at different temperatures, Hoechst 33258 is a suitable probe for the detection of double-stranded DNA. Thus, it is possible to monitor the concentration of double-stranded DNA in a suspension by measuring the total fluorescence intensity. The fluorescence denaturation profiles of DNA (calf thymus) were found to be comparable to absorption measurements. The decrease in fluorescence of metaphase chromosomes in suspension with increasing temperature may therefore be used to detect conformational changes of DNA in situ.
R K Schaller; R B Spiess; F F Bier; U Bettag; C Cremer
Related Documents :
15173119 - Dna mapping using microfluidic stretching and single-molecule detection of fluorescent ...
18711669 - Long, processive enzymatic dna synthesis using 100% dye-labeled terminal phosphate-link...
24308459 - Template-synthesized lignin nanotubes as vehicles for gene delivery into human cells.
15731389 - Orientational dynamics and dye-dna interactions in a dye-labeled dna aptamer.
2960249 - Quantitating small volumes of dilute dna samples containing sodium dodecyl sulfate.
15924389 - Probing the kinetics of sytox orange stain binding to double-stranded dna with implicat...
1206799 - Dna-minus temperature-sensitive mutants of adenovirus type 5 help adenovirus-associated...
9506889 - Formation and persistence of the miscoding dna alkylation product o6-ethylguanine in ma...
12376139 - Methodologies for bulky dna adduct analysis and biomonitoring of environmental and occu...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biophysical chemistry     Volume:  38     ISSN:  0301-4622     ISO Abbreviation:  Biophys. Chem.     Publication Date:  1990 Oct 
Date Detail:
Created Date:  1991-05-20     Completed Date:  1991-05-20     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0403171     Medline TA:  Biophys Chem     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  59-65     Citation Subset:  IM    
Institute of Applied Physics I, University of Heidelberg, F.R.G.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
DNA-Binding Proteins / chemistry*
Fluorescent Dyes
Protein Denaturation*
Spectrometry, Fluorescence
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Fluorescent Dyes; 23491-44-3/Bisbenzimidazole

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The presence of pregnancy-associated plasma protein-A in human corpora lutea: cellular and subcellul...
Next Document:  Quantitative IR spectrophotometry of peptide compounds in water (H2O) solutions. II. Amide absorptio...