Document Detail


Denaturation behaviour of DNA-protein-complexes detected in situ in metaphase chromosomes in suspension by Hoechst 33258 fluorescence.
MedLine Citation:
PMID:  1707689     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The denaturation behaviour of DNA-protein complexes in metaphase chromosomes in suspension was analysed in situ by Hoechst 33258 fluorescence. The results indicate that due to the stability of the dye molecule and the product of the molecular extinction coefficient and the quantum yield at different temperatures, Hoechst 33258 is a suitable probe for the detection of double-stranded DNA. Thus, it is possible to monitor the concentration of double-stranded DNA in a suspension by measuring the total fluorescence intensity. The fluorescence denaturation profiles of DNA (calf thymus) were found to be comparable to absorption measurements. The decrease in fluorescence of metaphase chromosomes in suspension with increasing temperature may therefore be used to detect conformational changes of DNA in situ.
Authors:
R K Schaller; R B Spiess; F F Bier; U Bettag; C Cremer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biophysical chemistry     Volume:  38     ISSN:  0301-4622     ISO Abbreviation:  Biophys. Chem.     Publication Date:  1990 Oct 
Date Detail:
Created Date:  1991-05-20     Completed Date:  1991-05-20     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0403171     Medline TA:  Biophys Chem     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  59-65     Citation Subset:  IM    
Affiliation:
Institute of Applied Physics I, University of Heidelberg, F.R.G.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bisbenzimidazole
Cattle
Chromosomes*
DNA-Binding Proteins / chemistry*
Fluorescent Dyes
Metaphase
Protein Denaturation*
Spectrometry, Fluorescence
Temperature
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Fluorescent Dyes; 23491-44-3/Bisbenzimidazole

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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