Document Detail

Demonstration of a surface antigen of Clostridium tyrobutyricum by use of immunoblotting with a monoclonal antibody.
MedLine Citation:
PMID:  2325579     Owner:  NLM     Status:  MEDLINE    
A monoclonal antibody, prepared against whole cells of Clostridium tyrobutyricum, recognized a surface antigen extracted by heat treatment or by hot phenol-water treatment. This antigen, after analysis by polyacrylamide gel electrophoresis and immunoblotting, has been shown to present a regularly-spaced ladder pattern similar to those shown by the lipopolysaccharide of many gram-negative bacteria. The proteinase K has been shown to have no effect on the recognition of this epitope by the monoclonal antibody. On the contrary, the inhibition of the antigen reactivity to the monoclonal antibody after a mild periodate oxidation suggests the involvement of a carbohydrate moiety in the epitope. Moreover, the SDS-PAGE analysis of phenol-water extracts has shown an additional compound, detected by silver staining but not recognized by the monoclonal antibody.
F Gueguen; G Robreau; F Talbot; R Malcoste
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Microbiology and immunology     Volume:  34     ISSN:  0385-5600     ISO Abbreviation:  Microbiol. Immunol.     Publication Date:  1990  
Date Detail:
Created Date:  1990-05-14     Completed Date:  1990-05-14     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7703966     Medline TA:  Microbiol Immunol     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  55-64     Citation Subset:  IM    
Université de Bretagne Occidentale, Institut Universitaire de Technologie de Quimper, France.
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MeSH Terms
Antibodies, Monoclonal
Antigens, Bacterial / analysis*
Antigens, Surface / analysis*
Blotting, Western / methods
Cell Wall / immunology
Clostridium / immunology*
Enzyme-Linked Immunosorbent Assay
Hot Temperature
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, Bacterial; 0/Antigens, Surface; 0/Phenols

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