Document Detail


Demonstration of a surface antigen of Clostridium tyrobutyricum by use of immunoblotting with a monoclonal antibody.
MedLine Citation:
PMID:  2325579     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A monoclonal antibody, prepared against whole cells of Clostridium tyrobutyricum, recognized a surface antigen extracted by heat treatment or by hot phenol-water treatment. This antigen, after analysis by polyacrylamide gel electrophoresis and immunoblotting, has been shown to present a regularly-spaced ladder pattern similar to those shown by the lipopolysaccharide of many gram-negative bacteria. The proteinase K has been shown to have no effect on the recognition of this epitope by the monoclonal antibody. On the contrary, the inhibition of the antigen reactivity to the monoclonal antibody after a mild periodate oxidation suggests the involvement of a carbohydrate moiety in the epitope. Moreover, the SDS-PAGE analysis of phenol-water extracts has shown an additional compound, detected by silver staining but not recognized by the monoclonal antibody.
Authors:
F Gueguen; G Robreau; F Talbot; R Malcoste
Related Documents :
12202579 - Detection of rickettsia prowazekii in body lice and their feces by using monoclonal ant...
22222459 - Leveraging sbdd in protein therapeutic development: antibody engineering.
6179659 - Enzyme-linked immunospecific assays for human alphafetoprotein using monoclonal antibod...
8467999 - Production and characterisation of a legionella pneumophila specific monoclonal antibody.
22618529 - Interference in antiphospholipid antibody assays.
2361359 - Pyruvate kinase isoenzymes in marine invertebrates: a comparative study by the use of m...
3500309 - The alloantibody response in the allogeneically pregnant rat. v. absence of cell-mediat...
6112249 - Antifolate actions of sulfasalazine on intact lymphocytes.
6156449 - Mechanism of interferon action: simian virus 40-specific early polypeptides synthesized...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Microbiology and immunology     Volume:  34     ISSN:  0385-5600     ISO Abbreviation:  Microbiol. Immunol.     Publication Date:  1990  
Date Detail:
Created Date:  1990-05-14     Completed Date:  1990-05-14     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7703966     Medline TA:  Microbiol Immunol     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  55-64     Citation Subset:  IM    
Affiliation:
Université de Bretagne Occidentale, Institut Universitaire de Technologie de Quimper, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal
Antigens, Bacterial / analysis*
Antigens, Surface / analysis*
Blotting, Western / methods
Cell Wall / immunology
Clostridium / immunology*
Enzyme-Linked Immunosorbent Assay
Hot Temperature
Phenols
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, Bacterial; 0/Antigens, Surface; 0/Phenols

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Granuloma-forming activity and antitumor activity of newly isolated mycoloyl glycolipid from Rhodoco...
Next Document:  Cell surface of a tetrads-forming mutant of Micrococcus luteus: chemical treatment of the cells and ...