Document Detail

Demonstration and quantification of "hyperchromic" erythrocytes by haematological analysers. Application to screening for hereditary and acquired spherocytosis.
MedLine Citation:
PMID:  11934218     Owner:  NLM     Status:  MEDLINE    
The double laser beam diffraction of spherized RBC used in the ADVIA 120 haematological analyser allows quantitation of cells aberrant not only by their volume but also by their haemoglobin concentration. The present investigation provides arguments for the identification of hyperchromic RBC as spherocytes, mainly the close relation between % hyperchromic cells and % lysed by the cryohaemolysis test. The percentage of hyperchromic erythrocytes may no longer be considered an instrumental artefact. Without allowing a definite diagnosis of hereditary spherocytosis, an increased percentage of hyperchromic cells indicates the degree of spherocytosis, making it an excellent automated and cost-free screening parameter for inherited and acquired corpuscular haemolysis.
Dolphe Kutter; Nathalie Coulon; Fernand Stirn; Martine Thoma; Jerzy Janecki
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical laboratory     Volume:  48     ISSN:  1433-6510     ISO Abbreviation:  Clin. Lab.     Publication Date:  2002  
Date Detail:
Created Date:  2002-04-05     Completed Date:  2003-07-11     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9705611     Medline TA:  Clin Lab     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  163-70     Citation Subset:  IM    
Laboratoires réunis, Junglinster, Grand Duchy of Luxembourg.
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MeSH Terms
Anemia, Hemolytic / diagnosis,  pathology
Erythrocyte Count / instrumentation,  standards
Erythrocytes, Abnormal / cytology*
Hematologic Tests / instrumentation
Spectrum Analysis
Spherocytes / cytology*
Spherocytosis, Hereditary / diagnosis,  pathology

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