| Demonstration of a proliferation regulatory factor (PRF) in normal human urine, and its purification. | |
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MedLine Citation:
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PMID: 6208186 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A material(s) affecting the proliferation of eukaryotic cells, such as C3H2K cells, and prokaryotic cells, such as E. coli, was found in the urine of normal healthy men and was concentrated in a certain fraction, "lysin-Sepharose eluate," of the urine. The material was named proliferation regulatory factor, PRF. With increase in the concentration of PRF, the proliferation rate of normal eukaryotes and prokaryotes increased to maxima of no more than twice those of untreated cells. On further increase in the concentration of PRF the growth rates of these cells decreased to the rates of untreated cells. PRF enhanced the rates of synthesis of DNA and RNA in C3H2K cells about 2-fold, but strongly suppressed the rates of growth, DNA synthesis, and RNA synthesis of cancerous cells, such as HeLa and MH134/M cells. PRF was purified from the "lysine-Sepharose eluate" by DEAE-cellulose column chromatography and gel filtration on a Sephadex G-100 column. PRF consisted of three molecules with molecular weights of 41,000, 7,800, and 5,100, which were named PRF-I, II, and III, respectively. The lysine-Sepharose eluate and purified PRF's had similar effects on the proliferation of eukaryotes and prokaryotes. |
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Authors:
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S Mori; T Onishi; M Muramatu |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of biochemistry Volume: 96 ISSN: 0021-924X ISO Abbreviation: J. Biochem. Publication Date: 1984 Jul |
Date Detail:
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Created Date: 1984-11-28 Completed Date: 1984-11-28 Revised Date: 2007-12-19 |
Medline Journal Info:
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Nlm Unique ID: 0376600 Medline TA: J Biochem Country: JAPAN |
Other Details:
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Languages: eng Pagination: 41-50 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Animals Cell Division / drug effects* Cells, Cultured Chromatography, Gel Chromatography, Ion Exchange DNA / biosynthesis DNA, Neoplasm / biosynthesis Escherichia coli / drug effects, growth & development Eukaryotic Cells / drug effects Growth Substances / isolation & purification, pharmacology, urine* Hela Cells / drug effects Humans Liver Neoplasms, Experimental / metabolism Male Mice Mice, Inbred C3H Proteins RNA / biosynthesis RNA, Neoplasm / biosynthesis |
| Chemical | |
Reg. No./Substance:
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0/DNA, Neoplasm; 0/Growth Substances; 0/Proteins; 0/RNA, Neoplasm; 0/proliferation regulatory factors, human urine; 63231-63-0/RNA; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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