Document Detail


Demonstration of a proliferation regulatory factor (PRF) in normal human urine, and its purification.
MedLine Citation:
PMID:  6208186     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A material(s) affecting the proliferation of eukaryotic cells, such as C3H2K cells, and prokaryotic cells, such as E. coli, was found in the urine of normal healthy men and was concentrated in a certain fraction, "lysin-Sepharose eluate," of the urine. The material was named proliferation regulatory factor, PRF. With increase in the concentration of PRF, the proliferation rate of normal eukaryotes and prokaryotes increased to maxima of no more than twice those of untreated cells. On further increase in the concentration of PRF the growth rates of these cells decreased to the rates of untreated cells. PRF enhanced the rates of synthesis of DNA and RNA in C3H2K cells about 2-fold, but strongly suppressed the rates of growth, DNA synthesis, and RNA synthesis of cancerous cells, such as HeLa and MH134/M cells. PRF was purified from the "lysine-Sepharose eluate" by DEAE-cellulose column chromatography and gel filtration on a Sephadex G-100 column. PRF consisted of three molecules with molecular weights of 41,000, 7,800, and 5,100, which were named PRF-I, II, and III, respectively. The lysine-Sepharose eluate and purified PRF's had similar effects on the proliferation of eukaryotes and prokaryotes.
Authors:
S Mori; T Onishi; M Muramatu
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biochemistry     Volume:  96     ISSN:  0021-924X     ISO Abbreviation:  J. Biochem.     Publication Date:  1984 Jul 
Date Detail:
Created Date:  1984-11-28     Completed Date:  1984-11-28     Revised Date:  2007-12-19    
Medline Journal Info:
Nlm Unique ID:  0376600     Medline TA:  J Biochem     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  41-50     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Animals
Cell Division / drug effects*
Cells, Cultured
Chromatography, Gel
Chromatography, Ion Exchange
DNA / biosynthesis
DNA, Neoplasm / biosynthesis
Escherichia coli / drug effects,  growth & development
Eukaryotic Cells / drug effects
Growth Substances / isolation & purification,  pharmacology,  urine*
Hela Cells / drug effects
Humans
Liver Neoplasms, Experimental / metabolism
Male
Mice
Mice, Inbred C3H
Proteins
RNA / biosynthesis
RNA, Neoplasm / biosynthesis
Chemical
Reg. No./Substance:
0/DNA, Neoplasm; 0/Growth Substances; 0/Proteins; 0/RNA, Neoplasm; 0/proliferation regulatory factors, human urine; 63231-63-0/RNA; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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