Document Detail

Demonstration and partial characterization of an atypical protein in the urine of a patient with primary amyloidosis.
MedLine Citation:
PMID:  6776783     Owner:  NLM     Status:  MEDLINE    
A paraprotein has been isolated from the urine of a patient with primary amyloidosis. Immunologically it was classified as a free lambda light chain. The molecular weight was 22500 daltons. N-terminal amino acid analysis demonstrated homology with lambda IV variable subgroup in 19 of the first 20 amino acids. Extensive homology with lambda IV chains was demonstrated also in the hypervariable region of the light chain. An antiserum produced against the paraprotein was rendered idiotype-specific by absorption with pooled human light chains. This antiserum stained tissue specimen from the rectum and liver of the patient by the indirect immunofluorescence technique. This strongly indicates that the free lambda light chains that can be isolated from the urine are also deposited in the tissues as amyloid substance.
L E Wille; E Olsen; O Førre; K Sletten; H Jentoft
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Publication Detail:
Type:  Case Reports; Journal Article    
Journal Detail:
Title:  Acta medica Scandinavica     Volume:  208     ISSN:  0001-6101     ISO Abbreviation:  Acta Med Scand     Publication Date:  1980  
Date Detail:
Created Date:  1981-01-29     Completed Date:  1981-01-29     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0370330     Medline TA:  Acta Med Scand     Country:  SWEDEN    
Other Details:
Languages:  eng     Pagination:  177-82     Citation Subset:  IM    
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MeSH Terms
Amino Acid Sequence
Amino Acids / analysis
Amyloidosis / urine*
Electrophoresis, Agar Gel
Enzyme-Linked Immunosorbent Assay / methods
Fluorescent Antibody Technique
Immunoglobulin Light Chains / isolation & purification
Immunoglobulin lambda-Chains / isolation & purification
Middle Aged
Molecular Weight
Paraproteins / immunology,  isolation & purification,  urine*
Reg. No./Substance:
0/Amino Acids; 0/Immunoglobulin Light Chains; 0/Immunoglobulin lambda-Chains; 0/Paraproteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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