| Demonstration and partial characterization of an atypical protein in the urine of a patient with primary amyloidosis. | |
| | |
MedLine Citation:
|
PMID: 6776783 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A paraprotein has been isolated from the urine of a patient with primary amyloidosis. Immunologically it was classified as a free lambda light chain. The molecular weight was 22500 daltons. N-terminal amino acid analysis demonstrated homology with lambda IV variable subgroup in 19 of the first 20 amino acids. Extensive homology with lambda IV chains was demonstrated also in the hypervariable region of the light chain. An antiserum produced against the paraprotein was rendered idiotype-specific by absorption with pooled human light chains. This antiserum stained tissue specimen from the rectum and liver of the patient by the indirect immunofluorescence technique. This strongly indicates that the free lambda light chains that can be isolated from the urine are also deposited in the tissues as amyloid substance. |
| | |
Authors:
|
L E Wille; E Olsen; O Førre; K Sletten; H Jentoft |
Related Documents
:
|
12371753 - Characterization of trans-monounsaturated alkenyl chains in total plasmalogens (1-o-alk... 6414463 - Dual role of a single multienzyme complex in the oxidative decarboxylation of pyruvate ... 4628623 - Localization and interconversion of tetrahydropteroylglutamates in isolated pea mitocho... |
Publication Detail:
|
Type: Case Reports; Journal Article |
Journal Detail:
|
Title: Acta medica Scandinavica Volume: 208 ISSN: 0001-6101 ISO Abbreviation: Acta Med Scand Publication Date: 1980 |
Date Detail:
|
Created Date: 1981-01-29 Completed Date: 1981-01-29 Revised Date: 2005-11-17 |
Medline Journal Info:
|
Nlm Unique ID: 0370330 Medline TA: Acta Med Scand Country: SWEDEN |
Other Details:
|
Languages: eng Pagination: 177-82 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Sequence Amino Acids / analysis Amyloidosis / urine* Electrophoresis, Agar Gel Enzyme-Linked Immunosorbent Assay / methods Fluorescent Antibody Technique Humans Immunoglobulin Light Chains / isolation & purification Immunoglobulin lambda-Chains / isolation & purification Male Middle Aged Molecular Weight Paraproteins / immunology, isolation & purification, urine* |
| Chemical | |
Reg. No./Substance:
|
0/Amino Acids; 0/Immunoglobulin Light Chains; 0/Immunoglobulin lambda-Chains; 0/Paraproteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Influence of diphenylhydantoin on lysosomal enzyme release during bone resorption in vitro.
Next Document: Cerebral metabolism following brain injury. I. Acid-base and pO2 changes.