Document Detail


Demonstration of mitochondrial oestrogen receptor beta and oestrogen-induced attenuation of cytochrome c oxidase subunit I expression in human periodontal ligament cells.
MedLine Citation:
PMID:  17223066     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: Periodontal ligament (PDL) cells express oestrogen receptor beta (ERbeta) protein, but cellular functions regulated by ERbeta in these cells have not been identified. In this study we determine if ERbeta is localised to mitochondria and if oestrogen regulates mitochondrial function in human PDL cells obtained from teeth extracted for orthodontic reasons.
DESIGN: Subcellular distribution of ERbeta was determined by confocal microscopy of cells co-stained with ERbeta antibody and the mitochondrion-selective probe MitoTracker and by immunogold electron microscopy. Expression of the mitochondrial enzyme cytochrome c oxidase subunit I, involved in oxidative phosphorylation, was determined by Western blotting in cells treated with or without physiological concentrations of the endogenous oestrogen 17beta-oestradiol.
RESULTS: ERbeta immunoreactivity was observed both in the nuclei and the cytoplasm. MitoTracker-labelling was observed in the cytoplasm, especially in the perinuclear region, but not in the nuclei. Co-localisation of ERbeta and MitoTracker was observed in cells derived from both male and female subjects. Mitochondrial localisation of ERbeta was confirmed by immunogold electron microscopy. Cells treated with or without 17beta-oestradiol (100 nM) displayed an identical pattern of staining for mitochondria. Treatment with 100 nM 17beta-oestradiol attenuated cytochrome c oxidase subunit I expression by about 30%, while combined treatment with 17beta-oestradiol and the ER blocker ICI 182780 (10 microM) had no effect.
CONCLUSION: This study demonstrates mitochondrial localisation of ERbeta and oestrogen-induced decrease in the expression of cytochrome c oxidase subunit I in human PDL cells, suggesting that oestrogen probably via ERbeta influences mitochondrial function and PDL cell energy metabolism.
Authors:
Daniel Jönsson; Jenny Nilsson; Malin Odenlund; Gunilla Bratthall; Jonas Broman; Eva Ekblad; Marie-Louise Lydrup; Bengt-Olof Nilsson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-01-16
Journal Detail:
Title:  Archives of oral biology     Volume:  52     ISSN:  0003-9969     ISO Abbreviation:  Arch. Oral Biol.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-05-09     Completed Date:  2007-12-18     Revised Date:  2013-06-11    
Medline Journal Info:
Nlm Unique ID:  0116711     Medline TA:  Arch Oral Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  669-76     Citation Subset:  D; IM    
Affiliation:
Department of Experimental Medical Science, Lund University, BMC F12, SE-221 84 Lund, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Aldehydes / diagnostic use
Cell Nucleus / ultrastructure
Cytoplasm / ultrastructure
Electron Transport Complex IV / antagonists & inhibitors*
Estradiol / analogs & derivatives,  pharmacology*
Estrogen Antagonists / pharmacology
Estrogen Receptor beta / analysis*
Estrogens / pharmacology*
Female
Humans
Immunohistochemistry
Male
Microscopy, Confocal
Microscopy, Immunoelectron
Mitochondria / enzymology,  ultrastructure*
Oxidative Phosphorylation
Periodontal Ligament / cytology,  drug effects,  ultrastructure*
Chemical
Reg. No./Substance:
0/Aldehydes; 0/Estrogen Antagonists; 0/Estrogen Receptor beta; 0/Estrogens; 0/mitotracker green FM; 22X328QOC4/fulvestrant; 50-28-2/Estradiol; EC 1.9.3.1/Electron Transport Complex IV

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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