Document Detail

Demonstration of a mechanism of aneuploidy in human oocytes using Multifluor fluorescence in situ hybridization.
MedLine Citation:
PMID:  11591425     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To evaluate the potential of Multifluor fluorescence in situ hybridization (M-FISH) for karyotyping the human oocyte and first polar body. DESIGN: Prospective case study. SETTING: Research laboratories, university hospital. PATIENT(S): A 33-year-old woman with polycystic ovary syndrome who was undergoing ovarian stimulation and ICSI. MAIN OUTCOME MEASURE(S): Karyotyping of all chromosomes within an oocyte and first polar body, using GV stage oocytes matured to metaphase II in vitro. RESULT(S): Oocyte hyperploidy was diagnosed by M-FISH to be 23, X +15 cht +19 cht +22 cht. The correspond- ing polar body was hypoploid, with a karyotype of 23, X -15 cht -19 cht -22 cht. This was due to unbalanced predivision at meiosis I. Reprobing confirmed karyotype assignments for chromosomes X, 13, 18, and 21. CONCLUSION(S): The mechanism involved in maternally derived aneuploidy can be defined by using M-FISH to simultaneously karyotype both oocyte and first polar body chromosomes at metaphase II. Multifluor FISH may be useful for investigative studies of maternally derived aneuploidy, which is a major cause of preimplantation waste in natural and assisted reproduction.
J M Clyde; R G Gosden; A J Rutherford; H M Picton
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Publication Detail:
Type:  Case Reports; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Fertility and sterility     Volume:  76     ISSN:  0015-0282     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2001 Oct 
Date Detail:
Created Date:  2001-10-09     Completed Date:  2001-12-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  837-40     Citation Subset:  IM    
Reproductive Biology Group, Academic Unit of Paediatrics, Obstetrics and Gynaecology, University of Leeds, Leeds, United Kingdom.
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MeSH Terms
In Situ Hybridization, Fluorescence*
Oocytes / physiology*
Prospective Studies

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