Document Detail


Demonstration in vitro that eucaryotic initiation factor 3 is active but that a cap-binding protein complex is inactive in poliovirus-infected HeLa cells.
MedLine Citation:
PMID:  6088805     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Protein synthesis initiation factor preparations from poliovirus-infected HeLa cells have reduced ability to initiate translation on capped mRNA. The defect in initiation factors has been variously attributed to inactivation of eucaryotic initiation factor 3 (eIF3), eIF4B, or a cap-binding protein (CBP) complex. We have developed a series of in vitro protein synthesis assays to show that eIF3 is active but a CBP complex activity is inactivated after poliovirus infection. eIF3 activity, when determined in the presence of purified CBP complex, is present in sucrose gradients of factors from both infected and uninfected cells. CBP complex activity, determined in the presence of eIF3 from poliovirus-infected cells, is present in uninfected cells only and comigrates on sucrose gradient with an activity which restores the ability of crude initiation factors from infected cells to translate capped globin mRNA. This is the first demonstration by a fractionated translation system that an activity which is attributable to CBP complex is inactivated in poliovirus-infected cells. The results also indicate that eIF3 is undetectable or has greatly reduced activity in the absence of CBP complex.
Authors:
D Etchison; J Hansen; E Ehrenfeld; I Edery; N Sonenberg; S Milburn; J W Hershey
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of virology     Volume:  51     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1984 Sep 
Date Detail:
Created Date:  1984-10-03     Completed Date:  1984-10-03     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  832-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Carrier Proteins / isolation & purification,  metabolism*
Cell Transformation, Viral*
Eukaryotic Initiation Factor-3
Hela Cells / metabolism
Humans
Kinetics
Molecular Weight
Peptide Initiation Factors / isolation & purification,  metabolism*
Poliovirus / genetics*
Protein Biosynthesis*
RNA Cap-Binding Proteins
Ribosomal Proteins / metabolism*
Grant Support
ID/Acronym/Agency:
AI-12387/AI/NIAID NIH HHS; AI-19921/AI/NIAID NIH HHS; GM-22135/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Eukaryotic Initiation Factor-3; 0/Peptide Initiation Factors; 0/RNA Cap-Binding Proteins; 0/Ribosomal Proteins
Comments/Corrections

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