| Demonstration of high opioid-like activity in isolated peptides from wheat gluten hydrolysates. | |
| | |
MedLine Citation:
|
PMID: 6099562 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Because of a possible relationship between schizophrenia and celiac disease, a condition in some individuals who are sensitive to wheat gluten proteins in the diet, there has been interest in observations that peptides derived from wheat gluten proteins exhibit opioid-like activity in in vitro tests. To determine the origin of the peptides exhibiting opioid activity, wheat proteins were fractionated by size (gel filtration), by charge differences (ion exchange chromatography) and by differences in hydrophobicity (reversed-phase HPLC). These fractions were hydrolyzed by pepsin or pepsin and trypsin and the resulting peptides separated by gel filtration chromatography. The separated peptides were tested for opioid-like activity by competitive binding to opioid receptor sites in rat brain tissue in the presence of tritium-labeled dihydromorphine. The peptides showed considerable differences in activity; while some peptides exhibited no activity, 0.5 mg of the most active peptides were equivalent to 1 nM of morphine in the binding assay. The most active peptides were derived from the gliadin fraction of the gluten complex. |
| | |
Authors:
|
F R Huebner; K W Lieberman; R P Rubino; J S Wall |
Publication Detail:
|
Type: Comparative Study; In Vitro; Journal Article |
Journal Detail:
|
Title: Peptides Volume: 5 ISSN: 0196-9781 ISO Abbreviation: Peptides Publication Date: 1984 Nov-Dec |
Date Detail:
|
Created Date: 1985-05-15 Completed Date: 1985-05-15 Revised Date: 2008-11-21 |
Medline Journal Info:
|
Nlm Unique ID: 8008690 Medline TA: Peptides Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 1139-47 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Brain / metabolism Celiac Disease / etiology Endorphins / isolation & purification*, metabolism Gliadin / metabolism Glutens / adverse effects, analysis* Humans Hydrolysis Male Peptides / isolation & purification*, pharmacology Plant Proteins / metabolism Rats Receptors, Opioid / metabolism Schizophrenia / etiology Triticum |
| Chemical | |
Reg. No./Substance:
|
0/Endorphins; 0/Peptides; 0/Plant Proteins; 0/Receptors, Opioid; 8002-80-0/Glutens; 9007-90-3/Gliadin; 9061-41-0/glutenin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Intracellular acetylation of desacetyl alpha MSH in the Xenopus laevis neurointermediate lobe.
Next Document: Ceruletide acts in the abdomen, not in the brain, to produce satiety.