Document Detail

Demonstration of gonadotropin releasing-hormone receptors on gonadotrophs and somatotrophs of the goldfish: an electron microscope study.
MedLine Citation:
PMID:  1667339     Owner:  NLM     Status:  MEDLINE    
Dispersed pituitary cells of the goldfish were incubated with biotinylated [D-Lys6, Pro9-N-ethylamide] salmon gonadotropin-releasing hormone (sGnRH-A) then avidingold (10 nm), and were fixed, embedded and sectioned. Cells were identified as gonadotrophs, somatotrophs, or prolactin cells using specific hormone antisera and protein-A gold (20 nm) as a marker. Attachment of the biotinylated sGnRH-A to the pituitary cell sections was determined by scanning cell surfaces for the smaller gold particles using the transmission electron microscope. Attachment was observed on gonadotrophs and somatotrophs, but was negligible on prolactin cells. Preincubation with unlabelled salmon gonadotropin-releasing hormone or chicken II gonadotropin-releasing hormone, or omission of the salmon gonadotropin-releasing hormone analog, prevented the reaction. The direct visualization of specific gonadotropin-releasing hormone receptors on gonadotrophs and somatotrophs supports the existence of direct stimulatory actions of gonadotropin-releasing hormone on gonadotropin and somatotropin release in gold-fish.
H Cook; J W Berkenbosch; M J Fernhout; K L Yu; R E Peter; J P Chang; J E Rivier
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Regulatory peptides     Volume:  36     ISSN:  0167-0115     ISO Abbreviation:  Regul. Pept.     Publication Date:  1991 Nov 
Date Detail:
Created Date:  1992-06-04     Completed Date:  1992-06-04     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8100479     Medline TA:  Regul Pept     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  369-78     Citation Subset:  IM    
Department of Zoology, University of Alberta, Edmonton, Canada.
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MeSH Terms
Chromatography, High Pressure Liquid
Microscopy, Electron
Pituitary Gland, Anterior / chemistry*,  ultrastructure
Receptors, LHRH / analysis*
Grant Support
Reg. No./Substance:
0/Receptors, LHRH

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