| Demonstration of feline infectious peritonitis virus in conjunctival epithelial cells from cats. A simple and reliable method for clinical veterinary virology screening. | |
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MedLine Citation:
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PMID: 2551353 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A simple technique demonstrating the presence of feline infectious peritonitis virus (FIPV) in cats is described. Smears from the third eyelid (membrana nictitans) were dried on microscope slides, fixed in aetone, and stained using an indirect immunofluorescence method with rabbit-anti-FIPV serum. By this method FIPV-antigen could be demonstrated in the cytoplasma of epithelial cells. The results obtained with this method (M3) were compared with serological and immunohistopathological methods for confirming FIPV-infection in cats. The results obtained by the M3 test were up to 85% in concordance with these other tests. Furthermore, the M3 test was easier and quicker to perform and should be well suited for clinical practice. |
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Authors:
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K Hök |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica Volume: 97 ISSN: 0903-4641 ISO Abbreviation: APMIS Publication Date: 1989 Sep |
Date Detail:
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Created Date: 1989-11-08 Completed Date: 1989-11-08 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8803400 Medline TA: APMIS Country: DENMARK |
Other Details:
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Languages: eng Pagination: 820-4 Citation Subset: IM |
Affiliation:
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Department of Medical Microbial Ecology, Karolinska Institutet, Stockholm, Sweden. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, Viral / analysis Cat Diseases / diagnosis* Cats Coronaviridae / immunology Coronaviridae Infections / diagnosis, veterinary* Epithelium / microbiology Fluorescent Antibody Technique Nictitating Membrane / microbiology* Peritonitis / diagnosis, veterinary* |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Viral |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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