Document Detail


Demonstration of axial elements of sex vesicles of spermatocytes using Coomassie brilliant blue.
MedLine Citation:
PMID:  6165100     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The axial element of sex chromosomes in the sex vesicle of rat and mouse spermatocytes has been visualized under the light microscope by the dye Coomassie brilliant blue (CBB). After staining in the CBB solution for 3-10 minutes, the axial elements appeared as darkly stained threads in the sex vesicles, whereas in controls stained with Giemsa or carbol fuchsin, the sex vesicles were usually uniformly stained. The axial elements are best seen when chromosome preparations were made by the flame drying technique. In rat spermatocytes the staining quality could be further improved by a brief treatment with trypsin solution (0.025%). The CBB staining procedure is simple and easily controllable. The results suggest that the CBB stained material is protein in nature and is more resistant to trypsin digestion than other nuclear proteins.
Authors:
H C Wang; B H Juurlink
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Stain technology     Volume:  56     ISSN:  0038-9153     ISO Abbreviation:  Stain Technol     Publication Date:  1981 Jan 
Date Detail:
Created Date:  1981-07-09     Completed Date:  1981-07-09     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0404535     Medline TA:  Stain Technol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  19-23     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Female
Male
Mice
Rats
Rosaniline Dyes*
Sex Chromosomes / ultrastructure*
Spermatocytes / ultrastructure*
Spermatozoa / ultrastructure*
Staining and Labeling
Trypsin
X Chromosome / ultrastructure*
Y Chromosome / ultrastructure*
Chemical
Reg. No./Substance:
0/Rosaniline Dyes; 6104-58-1/coomassie Brilliant Blue; EC 3.4.21.4/Trypsin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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