Document Detail

Demonstration of an associated anisotropy decay by frequency-domain fluorometry.
MedLine Citation:
PMID:  3663845     Owner:  NLM     Status:  MEDLINE    
We used frequency-domain fluorometry to demonstrate the presence of an associated decay of fluorescence anisotropy. In such systems the individual correlation times are associated with distinct emitting species, each with its own characteristic lifetime and rotational correlation times. We obtained an associated system using 1-anilino-8-naphthalenesulfonic acid (ANS) in the presence of increasing amounts of apomyoglobin. When both free and apomyoglobin-bound ANS contributed to the emission the differential polarized phase angles become negative at particular frequencies, even though the fundamental anisotropy (r0) is greater than zero. Additionally, the modulated anisotropy decreases at high frequencies. Both observations appear to be the unique consequence of an associated anisotropy decay, and are not possible for a multiexponential anisotropy decay of a single species.
H Szmacinski; R Jayaweera; H Cherek; J R Lakowicz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biophysical chemistry     Volume:  27     ISSN:  0301-4622     ISO Abbreviation:  Biophys. Chem.     Publication Date:  1987 Sep 
Date Detail:
Created Date:  1987-12-16     Completed Date:  1987-12-16     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0403171     Medline TA:  Biophys Chem     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  233-41     Citation Subset:  IM    
University of Maryland at Baltimore, School of Medicine, Department of Biological Chemistry 21201.
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MeSH Terms
Anilino Naphthalenesulfonates
Fluorescence Polarization / methods*
Fluorescent Dyes
Protein Binding
Time Factors
Grant Support
Reg. No./Substance:
0/Anilino Naphthalenesulfonates; 0/Apoproteins; 0/Fluorescent Dyes; 0/Myoglobin; 0/apomyoglobin; 82-76-8/1-anilino-8-naphthalenesulfonate

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