Document Detail

Demonstration of Anaplasma marginale in hemolymph of Dermacentor andersoni by animal inoculation and by fluorescent-antibody technique.
MedLine Citation:
PMID:  6346968     Owner:  NLM     Status:  MEDLINE    
Hemolymph was collected from adult Dermacentor andersoni Stiles that had been infected with Anaplasma marginale Theiler as nymphs. Before hemolymph was collected, the adult ticks were either incubated and unfed at 37 C for 2.5 days or fed for 6 days on sheep. Hemolymph collected from groups of 100 ticks was inoculated into susceptible splenectomized calves. Smears of hemolymph from the same groups of ticks were prepared for examination by fluorescent antibody technique. Hemolymph from incubated ticks caused anaplasmosis in 2 of 4 trials, and hemolymph from feeding ticks caused anaplasmosis in 4 of 4 trials. Moderately fluorescing bodies were demonstrated in some hemocytes from incubated ticks, whereas hemocytes from feeding ticks contained numerous clusters of brightly fluorescing bodies. Fluorescing bodies were not observed in hemocytes from control ticks.
K M Kocan; R D Oberst; S A Ewing; J A Hair; S J Barron
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  American journal of veterinary research     Volume:  44     ISSN:  0002-9645     ISO Abbreviation:  Am. J. Vet. Res.     Publication Date:  1983 May 
Date Detail:
Created Date:  1983-08-11     Completed Date:  1983-08-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375011     Medline TA:  Am J Vet Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  798-801     Citation Subset:  IM    
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MeSH Terms
Anaplasma / growth & development,  isolation & purification*
Blood Cells / microbiology*
Dermacentor / microbiology*
Fluorescent Antibody Technique / veterinary*
Hemocytes / microbiology*
Hemolymph / microbiology*
Ticks / microbiology*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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