Document Detail


Delta6-Desaturase (FADS2) deficiency unveils the role of omega3- and omega6-polyunsaturated fatty acids.
MedLine Citation:
PMID:  18685602     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Mammalian cell viability is dependent on the supply of the essential fatty acids (EFAs) linoleic and alpha-linolenic acid. EFAs are converted into omega3- and omega6-polyunsaturated fatty acids (PUFAs), which are essential constituents of membrane phospholipids and precursors of eicosanoids, anandamide and docosanoids. Whether EFAs, PUFAs and eicosanoids are essential for cell viability has remained elusive. Here, we show that deletion of Delta6-fatty acid desaturase (FADS2) gene expression in the mouse abolishes the initial step in the enzymatic cascade of PUFA synthesis. The lack of PUFAs and eicosanoids does not impair the normal viability and lifespan of male and female fads2-/- mice, but causes sterility. We further provide the molecular evidence for a pivotal role of PUFA-substituted membrane phospholipids in Sertoli cell polarity and blood-testis barrier, and the gap junction network between granulosa cells of ovarian follicles. The fads2-/- mouse is an auxotrophic mutant. It is anticipated that FADS2 will become a major focus in membrane, haemostasis, inflammation and atherosclerosis research.
Authors:
Wilhelm Stoffel; Barbara Holz; Britta Jenke; Erika Binczek; Robert Heinz Günter; Christine Kiss; Iakowos Karakesisoglou; Mario Thevis; Artur-Aron Weber; Stephan Arnhold; Klaus Addicks
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2008-8-07
Journal Detail:
Title:  The EMBO journal     Volume:  -     ISSN:  1460-2075     ISO Abbreviation:  EMBO J.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-8-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8208664     Medline TA:  EMBO J     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Center of Molecular Medicine (CMMC), Laboratory of Molecular Neurosciences, Institute of Biochemistry, University of Cologne, Cologne, Germany.
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