| Delta11 desaturases of Trichoplusia ni and Spodoptera littoralis exhibit dual catalytic behaviour. | |
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MedLine Citation:
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PMID: 17027848 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The Delta(11) desaturases found in moths such as Spodoptera littoralis play a critical role in the biosynthesis of their sex pheromones. The ability to functionally express these enzymes in yeast has allowed one to study the transformation of long-chain fatty acyl substrates to their 11-ene products in greater mechanistic detail. In this article, we report on the detection and quantitation of a minor 11-hydroxylated byproduct (0.1% of total fatty acids), which is formed by the Delta(11) desaturases found in Trichoplusia ni and Spodoptera littoralis. The position of the hydroxyl group was determined by characteristic mass spectral fragmentation of the trimethylsilyl derivatives and is in accord with predictions based on previous mechanistic investigations of the Spodoptera Delta(11) desaturase. The level of 11-hydroxylation was insensitive to the mode of desaturase expression (constitutive vs. induced) and the presence or absence of a b5-fusion domain. Our findings suggest that in future, a search for hydroxylated products should be included in functional analyses of insect desaturase genes. |
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Authors:
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Montserrat Serra; Lewis T Gauthier; Gemma Fabrias; Peter H Buist |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-08-22 |
Journal Detail:
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Title: Insect biochemistry and molecular biology Volume: 36 ISSN: 0965-1748 ISO Abbreviation: Insect Biochem. Mol. Biol. Publication Date: 2006 Oct |
Date Detail:
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Created Date: 2006-10-09 Completed Date: 2006-12-15 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9207282 Medline TA: Insect Biochem Mol Biol Country: England |
Other Details:
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Languages: eng Pagination: 822-5 Citation Subset: IM |
Affiliation:
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Department of Biological Organic Chemistry, IIQAB-CSIC, Jordi Girona 18, 08034 Barcelona, Spain. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Genetically Modified / metabolism Fatty Acid Desaturases / genetics, metabolism* Fatty Acids / chemistry, metabolism Hydroxylation Insect Proteins / genetics, metabolism* Mass Spectrometry Moths / enzymology* Recombinant Fusion Proteins / metabolism Saccharomyces cerevisiae / genetics Spodoptera / enzymology* |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Insect Proteins; 0/Recombinant Fusion Proteins; EC 1.14.19.-/Fatty Acid Desaturases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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