Document Detail

Delta-like 1 expression promotes goblet cell differentiation in Notch-inactivated human colonic epithelial cells.
MedLine Citation:
PMID:  20170633     Owner:  NLM     Status:  MEDLINE    
Notch signaling has previously been implicated in the regulation of the cell fate of intestinal epithelial cells. However, the expression and function of Notch ligands in the human intestine remain largely unknown. In the present study, we showed that Notch ligands Delta-like 1 (Dll1) and Delta-like 4 (Dll4) are expressed in a goblet cell-specific manner in human colonic tissue. Additionally, we found that Dll1 and Dll4 expression was regulated in-parallel with Atoh1 and MUC2, which are both under the control of the Notch-Hes1 signaling pathway. Because knockdown of Dll1 expression completely abrogated the acquisition of the goblet cell phenotype in Notch-inactivated colonic epithelial cells, we postulate that Dll1 might function as a cis-acting regulatory element that induces undifferentiated cells to become goblet cells. Our results suggest a link between Dll1 expression and human goblet cell differentiation that might be mediated by a function that is distinct from its role as a Notch receptor ligand.
Junko Akiyama; Ryuichi Okamoto; Michiko Iwasaki; Xiu Zheng; Shiro Yui; Kiichiro Tsuchiya; Tetsuya Nakamura; Mamoru Watanabe
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-02-17
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  393     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-03-22     Completed Date:  2010-04-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  662-7     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Inc. All rights reserved.
Department of Gastroenterology and Hepatology, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
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MeSH Terms
Basic Helix-Loop-Helix Transcription Factors / metabolism
Cell Differentiation*
Cells, Cultured
Colon / cytology*,  metabolism
Gene Knockdown Techniques
Goblet Cells / cytology*,  metabolism
Homeodomain Proteins / metabolism
Intercellular Signaling Peptides and Proteins / biosynthesis*,  genetics
Membrane Proteins / biosynthesis*,  genetics
Receptors, Notch / genetics,  metabolism
Signal Transduction
Reg. No./Substance:
0/Basic Helix-Loop-Helix Transcription Factors; 0/DLK1 protein, human; 0/Homeodomain Proteins; 0/Intercellular Signaling Peptides and Proteins; 0/Membrane Proteins; 0/Receptors, Notch; 149348-15-2/HES1 protein, human

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