Document Detail

Delivery of plasmid DNA to glial cells using pH-sensitive immunoliposomes.
MedLine Citation:
PMID:  8003028     Owner:  NLM     Status:  MEDLINE    
Immunoliposomes were constructed with an antibody specific to glial cells. They were used to examine the specificity and efficacy of cell type plasmid transfection. Liposomes contained a beta-galactosidase gene under control of an SV-40 promotor. Two different monoclonal antibodies of a different subclass, IgM and IgG, were examined for their targeting ability using immunoliposomes. Cultured C6 glioma (specific target cell type) and NIH 3T3 (control cell type, fibroblast) cells were transfected using these immunoliposomes. Results indicate a three-fold increase in transfection by the glial specific immunoliposomes, "gliasomes", in glial cell culture over control liposomes. Gliasomes were exposed to NIH 3T3 cells and showed no enhanced transfection over control liposomes. Gliasomes were tested for their specificity by the addition of excess antibody to the cell culture in order to saturate specific receptors on C6 glioma cells. Results indicate a reduced transfection, nearly three-fold, in cells that were saturated with excess antibody prior to exposure to the immunoliposomes.
E G Holmberg; Q R Reuer; E E Geisert; J L Owens
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  201     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1994 Jun 
Date Detail:
Created Date:  1994-07-14     Completed Date:  1994-07-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  888-93     Citation Subset:  IM    
Department of Chemistry/Physics, University of Alaska Anchorage 99508.
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MeSH Terms
3T3 Cells
Animals, Newborn
Antibodies, Monoclonal
Cells, Cultured
DNA, Bacterial / administration & dosage*,  metabolism
Drug Carriers
Escherichia coli / enzymology
Glioma / metabolism*
Kidney Cortex / cytology
Neuroglia / metabolism*
Plasmids / administration & dosage*
Transfection / methods*
Tumor Cells, Cultured
beta-Galactosidase / biosynthesis,  genetics
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/DNA, Bacterial; 0/Drug Carriers; 0/Liposomes; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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