Document Detail


Delivery of molecules into cells using localized single cell electroporation on ITO micro-electrode based transparent chip.
MedLine Citation:
PMID:  22674171     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Single cell electroporation is one of the nonviral method which successfully allows transfection of exogenous macromolecules into individual living cell. We present localized cell membrane electroporation at single-cell level by using indium tin oxide (ITO) based transparent micro-electrodes chip with inverted microscope. A focused ion beam (FIB) technique has been successfully deployed to fabricate transparent ITO micro-electrodes with submicron gaps, which can generate more intense electric field to produce very localized cell membrane electroporation. In our approach, we have successfully achieved 0.93 μm or smaller electroporation region on the cell surface to inject PI (Propidium Iodide) dye into the cell with 60 % cell viability. This experiments successfully demonstrate the cell self-recover process from the injected PI dye intensity variation. Our localized cell membrane electroporation technique (LSCMEP) not only generates reversible electroporation process but also it provides a clear optical path for potentially monitoring/tracking of drugs to deliver in single cell level.
Authors:
Sheng-Chiech Chen; Tuhin Subhra Santra; Chia-Jung Chang; Tsung-Ju Chen; Pen-Cheng Wang; Fan-Gang Tseng
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-5
Journal Detail:
Title:  Biomedical microdevices     Volume:  -     ISSN:  1572-8781     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100887374     Medline TA:  Biomed Microdevices     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Institute of Nano Engineering and Microsystems, National Tsing Hua University, Hsinchu City, Taiwan.
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