| Delivery mode shapes the acquisition and structure of the initial microbiota across multiple body habitats in newborns. | |
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MedLine Citation:
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PMID: 20566857 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Upon delivery, the neonate is exposed for the first time to a wide array of microbes from a variety of sources, including maternal bacteria. Although prior studies have suggested that delivery mode shapes the microbiota's establishment and, subsequently, its role in child health, most researchers have focused on specific bacterial taxa or on a single body habitat, the gut. Thus, the initiation stage of human microbiome development remains obscure. The goal of the present study was to obtain a community-wide perspective on the influence of delivery mode and body habitat on the neonate's first microbiota. We used multiplexed 16S rRNA gene pyrosequencing to characterize bacterial communities from mothers and their newborn babies, four born vaginally and six born via Cesarean section. Mothers' skin, oral mucosa, and vagina were sampled 1 h before delivery, and neonates' skin, oral mucosa, and nasopharyngeal aspirate were sampled <5 min, and meconium <24 h, after delivery. We found that in direct contrast to the highly differentiated communities of their mothers, neonates harbored bacterial communities that were undifferentiated across multiple body habitats, regardless of delivery mode. Our results also show that vaginally delivered infants acquired bacterial communities resembling their own mother's vaginal microbiota, dominated by Lactobacillus, Prevotella, or Sneathia spp., and C-section infants harbored bacterial communities similar to those found on the skin surface, dominated by Staphylococcus, Corynebacterium, and Propionibacterium spp. These findings establish an important baseline for studies tracking the human microbiome's successional development in different body habitats following different delivery modes, and their associated effects on infant health. |
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Authors:
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Maria G Dominguez-Bello; Elizabeth K Costello; Monica Contreras; Magda Magris; Glida Hidalgo; Noah Fierer; Rob Knight |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-06-21 |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 107 ISSN: 1091-6490 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-07-09 Completed Date: 2010-08-11 Revised Date: 2010-12-30 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: United States |
Other Details:
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Languages: eng Pagination: 11971-5 Citation Subset: IM |
Affiliation:
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Department of Biology, University of Puerto Rico, San Juan, Puerto Rico 00931. maria.dominguez1@upr.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Bacteria / classification, genetics, isolation & purification* Base Sequence Cesarean Section DNA Primers / genetics Delivery, Obstetric / methods* Ecosystem Female Humans Infant, Newborn* Male Metagenome* / genetics Mouth Mucosa / microbiology Nasopharynx / microbiology Pregnancy RNA, Bacterial / genetics, isolation & purification RNA, Ribosomal, 16S / genetics, isolation & purification Skin / microbiology Vagina / microbiology Venezuela Young Adult |
| Grant Support | |
ID/Acronym/Agency:
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R01HG004872/HG/NHGRI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/RNA, Bacterial; 0/RNA, Ribosomal, 16S |
| Comments/Corrections | |
Comment In:
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Proc Natl Acad Sci U S A. 2010 Oct 19;107(42):E159; author reply E160
[PMID:
20876088
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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