Document Detail


Delivery mode shapes the acquisition and structure of the initial microbiota across multiple body habitats in newborns.
MedLine Citation:
PMID:  20566857     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Upon delivery, the neonate is exposed for the first time to a wide array of microbes from a variety of sources, including maternal bacteria. Although prior studies have suggested that delivery mode shapes the microbiota's establishment and, subsequently, its role in child health, most researchers have focused on specific bacterial taxa or on a single body habitat, the gut. Thus, the initiation stage of human microbiome development remains obscure. The goal of the present study was to obtain a community-wide perspective on the influence of delivery mode and body habitat on the neonate's first microbiota. We used multiplexed 16S rRNA gene pyrosequencing to characterize bacterial communities from mothers and their newborn babies, four born vaginally and six born via Cesarean section. Mothers' skin, oral mucosa, and vagina were sampled 1 h before delivery, and neonates' skin, oral mucosa, and nasopharyngeal aspirate were sampled <5 min, and meconium <24 h, after delivery. We found that in direct contrast to the highly differentiated communities of their mothers, neonates harbored bacterial communities that were undifferentiated across multiple body habitats, regardless of delivery mode. Our results also show that vaginally delivered infants acquired bacterial communities resembling their own mother's vaginal microbiota, dominated by Lactobacillus, Prevotella, or Sneathia spp., and C-section infants harbored bacterial communities similar to those found on the skin surface, dominated by Staphylococcus, Corynebacterium, and Propionibacterium spp. These findings establish an important baseline for studies tracking the human microbiome's successional development in different body habitats following different delivery modes, and their associated effects on infant health.
Authors:
Maria G Dominguez-Bello; Elizabeth K Costello; Monica Contreras; Magda Magris; Glida Hidalgo; Noah Fierer; Rob Knight
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-06-21
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  107     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-07-09     Completed Date:  2010-08-11     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  11971-5     Citation Subset:  IM    
Affiliation:
Department of Biology, University of Puerto Rico, San Juan, Puerto Rico 00931. maria.dominguez1@upr.edu
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MeSH Terms
Descriptor/Qualifier:
Adult
Bacteria / classification,  genetics,  isolation & purification*
Base Sequence
Cesarean Section
DNA Primers / genetics
Delivery, Obstetric / methods*
Ecosystem
Female
Humans
Infant, Newborn*
Male
Metagenome* / genetics
Mouth Mucosa / microbiology
Nasopharynx / microbiology
Pregnancy
RNA, Bacterial / genetics,  isolation & purification
RNA, Ribosomal, 16S / genetics,  isolation & purification
Skin / microbiology
Vagina / microbiology
Venezuela
Young Adult
Grant Support
ID/Acronym/Agency:
R01HG004872/HG/NHGRI NIH HHS
Chemical
Reg. No./Substance:
0/DNA Primers; 0/RNA, Bacterial; 0/RNA, Ribosomal, 16S
Comments/Corrections
Comment In:
Proc Natl Acad Sci U S A. 2010 Oct 19;107(42):E159; author reply E160   [PMID:  20876088 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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