| Delivery of genes to hematopoietic stem cells. | |
| | |
MedLine Citation:
|
PMID: 14970614 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Bone marrow hematopoiesis is maintained by hematopoietic stem cells (HSC). Because of their unique features to self-renew and differentiate along all lineages of hematopoietic cells, even a single HSC can completely reconstitute bone marrow hematopoiesis of irradiated recipients (2). Therefore, HSCs are considered to be the ideal target cell population in gene-therapy fields for genetic disorders that are susceptible to bone marrow transplantation (3). However, because most HSCs are quiescent, it is difficult to transduce them using retroviral vectors (4). Furthermore, retroviral vectors, especially Moloney murine leukemia virus (MMLV)-based retroviral vectors that have been commonly used in gene-therapy clinical trials, are very susceptible to de novo methylation in immature cells such as embryonic stem (ES) cells, embryonal carcinoma cells (EC), and HSCs, resulting in shut off/silencing of the transgene expression in vivo (5). This is another obstacle for successful gene delivery into HSCs. |
| | |
Authors:
|
Masafumi Onodera |
Related Documents
:
|
15140204 - The bone marrow is akin to skin: hcell and the biology of hematopoietic stem cell homing. 11499694 - Use of in vitro assays to assess hematotoxic effects of environmental compounds. 14726374 - Scl is required for normal function of short-term repopulating hematopoietic stem cells. 17709604 - Evidence for mpl w515l/k mutations in hematopoietic stem cells in primitive myelofibrosis. 11344354 - Normally suppressing cd40 coregulatory signals delivered by airway macrophages to th2 l... 21323594 - Stem cells and stroke: opportunities, challenges and strategies. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Methods in molecular biology (Clifton, N.J.) Volume: 246 ISSN: 1064-3745 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2004 |
Date Detail:
|
Created Date: 2004-02-18 Completed Date: 2004-03-19 Revised Date: 2006-04-21 |
Medline Journal Info:
|
Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
|
Languages: eng Pagination: 527-39 Citation Subset: IM |
Affiliation:
|
Department of Hematology, Institute of Clinical Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Line Gene Transfer Techniques* Genetic Vectors Hematopoietic Stem Cells / metabolism* Mice Retroviridae / genetics Transduction, Genetic Viral Structural Proteins / genetics |
| Chemical | |
Reg. No./Substance:
|
0/Viral Structural Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Retrovirus-mediated gene transfer to tumors: utilizing the replicative power of viruses to achieve h...
Next Document: Delivery and expression of heterologous genes in mammalian cells using self-replicating alphavirus v...