| Delivery of endothelial cells to balloon-dilated rabbit arteries with use of a local delivery catheter. | |
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MedLine Citation:
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PMID: 11340139 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: Experiments were performed to determine if a local delivery catheter could deliver endothelial cells that would be retained on the luminal surface of balloon-dilated arteries. MATERIALS AND METHODS: Six New Zealand White rabbits underwent carotid catheterization, arteriography, and balloon angioplasty of an external iliac artery. A local delivery catheter (Dispatch) was then positioned at the site of angioplasty and the 3-mm balloon was inflated. Cultured rabbit endothelial cells (1.26 +/- 0.3 x 10(6) ), previously stained with fluorescent dye PKH26, were delivered to the artery in three infusions separated by 10 minutes. The delivery balloon was deflated and removed 25 minutes after the last delivery. The arteries were then perfusion-fixed in situ at physiologic pressure, removed, and divided into four segments, and the segments were rapidly frozen and cryosectioned. Eight sections from each arterial segment were examined by means of epifluorescence microscopy. The luminal surface of each artery was visually divided into eight sectors of equal length and each sector was assigned a score based on the degree of endothelial coverage (0 = no coverage, 1 = <50% coverage, 2 = >50% coverage). RESULTS: The endothelial coverage score for the six arteries averaged 0.40 +/- 0.46 (SD; range, 0.04-1.24). Areas of each artery receiving scores of 0, 1, and 2 averaged 68%, 25%, and 7%, respectively. Average coverage scores were 0.42, 0.38, 0.51, and 0.28 for individual segments along the length of the artery. CONCLUSIONS: The Dispatch local delivery catheter is able to deliver endothelial cells that adhere to balloon-dilated arteries. Although the magnitude of cellular retention was modest and varied among arteries, the retention along the length of each artery was constant. |
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Authors:
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E Dillavou; P Cupp; P M Consigny |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of vascular and interventional radiology : JVIR Volume: 12 ISSN: 1051-0443 ISO Abbreviation: J Vasc Interv Radiol Publication Date: 2001 May |
Date Detail:
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Created Date: 2001-05-07 Completed Date: 2001-08-30 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9203369 Medline TA: J Vasc Interv Radiol Country: United States |
Other Details:
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Languages: eng Pagination: 601-5 Citation Subset: IM |
Affiliation:
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Department of Surgery, Thomas Jefferson University Hospital, Philadelphia, Pennsylvania, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Angiography Animals Balloon Dilatation / methods* Carotid Arteries Cell Separation Cell Survival Cells, Cultured Endothelium, Vascular / cytology* Iliac Artery Perfusion Rabbits |
| Grant Support | |
ID/Acronym/Agency:
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HL55508/HL/NHLBI NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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