Document Detail

Delivery of antisense oligonucleotides to neuroblastoma cells.
MedLine Citation:
PMID:  11043534     Owner:  NLM     Status:  MEDLINE    
pH-Sensitive liposomes composed of dioleoylphosphatidylethanolamine and cholesterol hemisuccinate (3:2 mol/mol) were applied in delivery of antisense oligodeoxynucleotides (asODN) into NG 108-15 neuroblastoma and glioma cells. Fluorescently labelled asODN were entrapped in liposomes by a modified freeze-thawing method (20% encapsulation efficiency). The uptake of asODN (free or entrapped in liposomes) by NG 108-15 cells was monitored by fluorescence-activated cell sorting and confocal microscopy. Delivery of asODN was significantly improved when antisense were entrapped in liposomes as compared to free (nonliposomal) asODN. The uptake was dose-dependent and optimum was achieved after 2 h incubation.
N Skalko-Basnet; M Tohda; H Watanabe
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Neuroreport     Volume:  11     ISSN:  0959-4965     ISO Abbreviation:  Neuroreport     Publication Date:  2000 Sep 
Date Detail:
Created Date:  2001-01-26     Completed Date:  2001-02-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9100935     Medline TA:  Neuroreport     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  3117-21     Citation Subset:  IM    
Department of Pharmacology, Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, Sugitani, Japan.
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MeSH Terms
Drug Delivery Systems*
Fluorescein-5-isothiocyanate / diagnostic use
Liposomes / chemistry,  pharmacokinetics*
Oligonucleotides, Antisense / pharmacokinetics*
Time Factors
Tumor Cells, Cultured / drug effects*
Reg. No./Substance:
0/Liposomes; 0/Oligonucleotides, Antisense; 3326-32-7/Fluorescein-5-isothiocyanate

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