Document Detail


Delivery of TFPI-2 using SonoVue and adenovirus results in the suppression of thrombosis and arterial re-stenosis.
MedLine Citation:
PMID:  20682602     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Genes could be used to treat atherosclerosis. The key problem is how to target a gene through the walls of arteries in free-flowing blood. TFPI-2 has been shown to suppress thrombosis and arterial re-stenosis, which indicates its potential function in gene therapy for atherosclerosis. The microbubble ultrasound contrast agent is widely applied in diagnostic imaging, and could be used for transferring genes into arteries. By transfecting TFPI-2 into arteries using SonoVue (a kind of microbubble ultrasound contrast agent), we identified TFPI-2 as an available factor for inhibiting the proliferation of vascular endothelial cells in vivo. Compared with adenovirus, SonoVue showed similar gene transfection efficiency, but the latter showed stronger inhibition of thrombosis and arterial re-stenosis with a high expression of TFPI-2 protein in vitro and in vivo. SonoVue was less damaging when transfecting genes into the arterial wall. These data indicate that transfecting human TFPI-2 into the arterial wall may suppress thrombosis and arterial re-stenosis, and reduce atherosclerosis.
Authors:
Yuxue Wang; Jie Zhou; Yanrong Zhang; Ximing Wang; Juan Chen
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-08-03
Journal Detail:
Title:  Experimental biology and medicine (Maywood, N.J.)     Volume:  235     ISSN:  1535-3699     ISO Abbreviation:  Exp. Biol. Med. (Maywood)     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-23     Completed Date:  2010-09-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100973463     Medline TA:  Exp Biol Med (Maywood)     Country:  England    
Other Details:
Languages:  eng     Pagination:  1072-81     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Molecular Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Adenoviridae / genetics*
Adenoviridae Infections / genetics
Arterioles / metabolism
Gene Therapy
Glycoproteins / genetics
Humans
Microbubbles
Phospholipids
Sulfur Hexafluoride
Thrombosis / genetics
Transfection
Chemical
Reg. No./Substance:
0/Glycoproteins; 0/Phospholipids; 0/contrast agent BR1; 0/tissue-factor-pathway inhibitor 2; 2551-62-4/Sulfur Hexafluoride

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