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Delivery of nucleic acids via disulfide-based carrier systems.
MedLine Citation:
PMID:  20882498     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Nucleic acids are not only expected to assume a pivotal position as "drugs" in the treatment of genetic and acquired diseases, but could also act as molecular cues to control the microenvironment during tissue regeneration. Despite this promise, the efficient delivery of nucleic acids to their side of action is still the major hurdle. One among many prerequisites for a successful carrier system for nucleic acids is high stability in the extracellular environment, accompanied by an efficient release of the cargo in the intracellular compartment. A promising strategy to create such an interactive delivery system is to exploit the redox gradient between the extra- and intracellular compartments. In this review, emphasis is placed on the biological rationale for the synthesis of redox sensitive, disulfide-based carrier systems, as well as the extra- and intracellular processing of macromolecules containing disulfide bonds. Moreover, the basic synthetic approaches for introducing disulfide bonds into carrier molecules, together with examples that demonstrate the benefit of disulfides at the individual stages of nucleic acid delivery, will be presented.
Authors:
Sonja Bauhuber; Constantin Hozsa; Miriam Breunig; Achim Göpferich
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Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Advanced materials (Deerfield Beach, Fla.)     Volume:  21     ISSN:  1521-4095     ISO Abbreviation:  Adv. Mater. Weinheim     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2010-09-30     Completed Date:  2010-12-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9885358     Medline TA:  Adv Mater     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  3286-306     Citation Subset:  -    
Affiliation:
Universität Regensburg, Germany.
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