Document Detail

Deletions and rearrangements in Kluyveromyces lactis mitochondrial DNA.
MedLine Citation:
PMID:  2692854     Owner:  NLM     Status:  MEDLINE    
Three classes of respiratory deficient mutants have been isolated from a fusant between Kluyveromyces lactis and Saccharomyces cerevisiae that contains only K. lactis mtDNA. One class (15 isolates), resemble rho 0 mutants of S. cerevisiae as they lack detectable mtDNA. A second class (16 isolates), resemble point mutations (mit-) or nuclear lesions (pet-) of S. cerevisiae as no detectable change is found in their mtDNA. The third class (five isolates), with deletions and rearrangements in their mtDNA are comparable to S. cerevisiae petite (rho-) mutants. Surprisingly, three of the five deletion mutants have lost the same 8.0 kb sector of the mtDNA that encompasses the entire cytochrome oxidase subunit 2 gene and the majority of the adjacent cytochrome oxidase subunit 1 gene. In the other strains, deletions are accompanied by complex rearrangements together with substoiciometric bands and in one instance an amplified sector of 800 bp. By contrast to G + C rich short direct repeats forming deletion sites in S. cerevisiae mtDNA, excision of the 8.0 kb sector in K. lactis mtDNA occurs at an 11 bp A + T rich direct repeat CTAATATATAT. The recovery of three strains manifesting this deletion suggests there are limited sites for intramolecular recombination leading to excision in K. lactis mtDNA.
C M Hardy; C L Galeotti; G D Clark-Walker
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Current genetics     Volume:  16     ISSN:  0172-8083     ISO Abbreviation:  Curr. Genet.     Publication Date:  1989 Dec 
Date Detail:
Created Date:  1990-03-15     Completed Date:  1990-03-15     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  8004904     Medline TA:  Curr Genet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  419-27     Citation Subset:  IM    
Molecular and Population Genetics Group, Research School of Biological Sciences, Australian National University, Canberra, A.C.T.
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MeSH Terms
Base Sequence
Chromosome Deletion*
Cytochromes / analysis
DNA, Fungal / genetics
DNA, Mitochondrial / genetics*
Gene Rearrangement*
Kluyveromyces / genetics*,  metabolism
Molecular Sequence Data
Repetitive Sequences, Nucleic Acid
Restriction Mapping
Saccharomyces cerevisiae / genetics
Saccharomycetales / genetics*
Reg. No./Substance:
0/Cytochromes; 0/DNA, Fungal; 0/DNA, Mitochondrial

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