Document Detail


Deletion mapping of the polA-metB region of the Escherichia coli chromosome.
MedLine Citation:
PMID:  35528     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A lambdacI857 prophage inserted into one of the genes of the rha locus was used to select deletions unambiguously ordering the markers polA-glnA-rha-pfkA-tpi-metBJF. Transduction with phage P1 indicates at least 70% linkage between glnA and polA. The order of the pfk and tpi markers is reversed from that previously published. Despite the relatively large distance separating the glnA and rha loci, deletions removing this entire region have no obvious phenotype. The isolation of Tn10 transposons integrated at different sites between rha and glnA greatly facilitated this work.
Authors:
G Pahel; F R Bloom; B Tyler
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  138     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1979 May 
Date Detail:
Created Date:  1979-07-28     Completed Date:  1979-07-28     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  653-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Chromosome Mapping
Escherichia coli / genetics*
Genes*
Glutamate-Ammonia Ligase / genetics*
Linkage (Genetics)
Mutation*
Recombination, Genetic
Rhamnose / genetics*
Transduction, Genetic
Chemical
Reg. No./Substance:
10485-94-6/Rhamnose; EC 6.3.1.2/Glutamate-Ammonia Ligase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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