| Deletion of cgR_1596 and cgR_2070, encoding NlpC/P60 proteins, causes a defect in cell separation in Corynebacterium glutamicum R. | |
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MedLine Citation:
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PMID: 18931118 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In previous work, random genome deletion mutants of Corynebacterium glutamicum R were generated using the insertion sequence (IS) element IS31831 and the Cre/loxP excision system. One of these mutants, C. glutamicum strain RD41, resulting from the deletion of a 10.1-kb genomic region (DeltacgR_1595 through cgR_1604) from the WT strain, showed cell elongation, and several lines appeared on the cell surface (bamboo shape). The morphological changes were suppressed by overexpression of cgR_1596. Single disruption of cgR_1596 in WT C. glutamicum R resulted in morphological changes similar to those observed in the RD41 strain. CgR_1596 has a predicted secretion signal peptide in the amino-terminal region and a predicted NlpC/P60 domain, which is conserved in cell wall hydrolases, in the carboxyl-terminal region. In C. glutamicum R, CgR_0802, CgR_1596, CgR_2069, and CgR_2070 have the NlpC/P60 domain; however, only simultaneous disruption of cgR_1596 and cgR_2070, and not cgR_2070 single disruption, resulted in cell growth delay and more severe morphological changes than disruption of cgR_1596. Transmission electron microscopy revealed multiple septa within individual cells of cgR_1596 single and cgR_1596-cgR_2070 double disruptants. Taken together, these results suggest that cgR_1596 and cgR_2070 are involved in cell separation and cell growth in C. glutamicum. |
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Authors:
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Yota Tsuge; Hidetaka Ogino; Haruhiko Teramoto; Masayuki Inui; Hideaki Yukawa |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-10-17 |
Journal Detail:
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Title: Journal of bacteriology Volume: 190 ISSN: 1098-5530 ISO Abbreviation: J. Bacteriol. Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2008-11-26 Completed Date: 2008-12-08 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: United States |
Other Details:
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Languages: eng Pagination: 8204-14 Citation Subset: IM |
Affiliation:
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Research Institute of Innovative Technology for the Earth, Kizugawa, Kyoto 619-0292, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Proteins
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genetics,
metabolism* Cell Wall / metabolism Corynebacterium glutamicum / drug effects, genetics, growth & development*, metabolism DNA, Bacterial / genetics Gene Deletion* Genes, Bacterial Genetic Complementation Test Hydrolases / genetics, metabolism* Microbial Sensitivity Tests Microscopy, Electron, Transmission Mutation Plasmids Protein Sorting Signals Transformation, Bacterial beta-Lactams / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/DNA, Bacterial; 0/Protein Sorting Signals; 0/beta-Lactams; EC 3.-/Hydrolases |
| Comments/Corrections | |
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