Document Detail


Deletion of cgR_1596 and cgR_2070, encoding NlpC/P60 proteins, causes a defect in cell separation in Corynebacterium glutamicum R.
MedLine Citation:
PMID:  18931118     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In previous work, random genome deletion mutants of Corynebacterium glutamicum R were generated using the insertion sequence (IS) element IS31831 and the Cre/loxP excision system. One of these mutants, C. glutamicum strain RD41, resulting from the deletion of a 10.1-kb genomic region (DeltacgR_1595 through cgR_1604) from the WT strain, showed cell elongation, and several lines appeared on the cell surface (bamboo shape). The morphological changes were suppressed by overexpression of cgR_1596. Single disruption of cgR_1596 in WT C. glutamicum R resulted in morphological changes similar to those observed in the RD41 strain. CgR_1596 has a predicted secretion signal peptide in the amino-terminal region and a predicted NlpC/P60 domain, which is conserved in cell wall hydrolases, in the carboxyl-terminal region. In C. glutamicum R, CgR_0802, CgR_1596, CgR_2069, and CgR_2070 have the NlpC/P60 domain; however, only simultaneous disruption of cgR_1596 and cgR_2070, and not cgR_2070 single disruption, resulted in cell growth delay and more severe morphological changes than disruption of cgR_1596. Transmission electron microscopy revealed multiple septa within individual cells of cgR_1596 single and cgR_1596-cgR_2070 double disruptants. Taken together, these results suggest that cgR_1596 and cgR_2070 are involved in cell separation and cell growth in C. glutamicum.
Authors:
Yota Tsuge; Hidetaka Ogino; Haruhiko Teramoto; Masayuki Inui; Hideaki Yukawa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-10-17
Journal Detail:
Title:  Journal of bacteriology     Volume:  190     ISSN:  1098-5530     ISO Abbreviation:  J. Bacteriol.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-11-26     Completed Date:  2008-12-08     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8204-14     Citation Subset:  IM    
Affiliation:
Research Institute of Innovative Technology for the Earth, Kizugawa, Kyoto 619-0292, Japan.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / genetics,  metabolism*
Cell Wall / metabolism
Corynebacterium glutamicum / drug effects,  genetics,  growth & development*,  metabolism
DNA, Bacterial / genetics
Gene Deletion*
Genes, Bacterial
Genetic Complementation Test
Hydrolases / genetics,  metabolism*
Microbial Sensitivity Tests
Microscopy, Electron, Transmission
Mutation
Plasmids
Protein Sorting Signals
Transformation, Bacterial
beta-Lactams / pharmacology
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA, Bacterial; 0/Protein Sorting Signals; 0/beta-Lactams; EC 3.-/Hydrolases
Comments/Corrections

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