Document Detail

Deletion of aceE gene (encoding a component of pyruvate dehydrogenase) attenuates Salmonella enterica serovar Enteritidis.
MedLine Citation:
PMID:  21707778     Owner:  NLM     Status:  Publisher    
Salmonella enterica serovar Enteritidis (S. Enteritidis) is a major food-borne pathogen. From a transposon insertion mutant library created previously using S. Enteritidis 10/02, one of the mutants was identified to have a 50% lethal dose (LD(50) ) at least 100 times that of the parental strain in young chicks, with an attenuation in a poorly studied gene encoding a component of pyruvate dehydrogenase, namely aceE gene. Evaluation of in vitro virulence characteristics of ΔaceE∷kan mutant revealed that it was less able to invade epithelial cells, less resistant to reactive oxygen intermediate (ROI), less able to survive within a chicken macrophage cell line, and had a retarded growth rate compared to parental strain. Young chicks vaccinated with 2×10(9) CFU of ΔaceE∷kan mutant were protected from subsequent challenge of parental strain, with the mutant colonized in the liver and spleen in a shorter time than the group infected with parental strain. In addition, compared to the parental strain, ΔaceE∷kan mutant did not cause persistence eggshell contamination of vaccinated hens.
Ervinna Pang; Chang Tien-Lin; Madhan Selvaraj; Jason Chang; Jimmy Kwang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-6-27
Journal Detail:
Title:  FEMS immunology and medical microbiology     Volume:  -     ISSN:  1574-695X     ISO Abbreviation:  -     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-6-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9315554     Medline TA:  FEMS Immunol Med Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
Animal Health Biotechnology Group, Temasek Life Sciences Laboratory, National University of Singapore, Singapore Department of Microbiology, Faculty of Medicine, National University of Singapore, Singapore.
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