Document Detail


Deletion of aceE gene (encoding a component of pyruvate dehydrogenase) attenuates Salmonella enterica serovar Enteritidis.
MedLine Citation:
PMID:  21707778     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Salmonella enterica serovar Enteritidis (S. Enteritidis) is a major food-borne pathogen. From a transposon insertion mutant library created previously using S. Enteritidis 10/02, one of the mutants was identified to have a 50% lethal dose (LD(50) ) at least 100 times that of the parental strain in young chicks, with an attenuation in a poorly studied gene encoding a component of pyruvate dehydrogenase, namely aceE gene. Evaluation of in vitro virulence characteristics of ΔaceE∷kan mutant revealed that it was less able to invade epithelial cells, less resistant to reactive oxygen intermediate (ROI), less able to survive within a chicken macrophage cell line, and had a retarded growth rate compared to parental strain. Young chicks vaccinated with 2×10(9) CFU of ΔaceE∷kan mutant were protected from subsequent challenge of parental strain, with the mutant colonized in the liver and spleen in a shorter time than the group infected with parental strain. In addition, compared to the parental strain, ΔaceE∷kan mutant did not cause persistence eggshell contamination of vaccinated hens.
Authors:
Ervinna Pang; Chang Tien-Lin; Madhan Selvaraj; Jason Chang; Jimmy Kwang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-6-27
Journal Detail:
Title:  FEMS immunology and medical microbiology     Volume:  -     ISSN:  1574-695X     ISO Abbreviation:  -     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-6-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9315554     Medline TA:  FEMS Immunol Med Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
Affiliation:
Animal Health Biotechnology Group, Temasek Life Sciences Laboratory, National University of Singapore, Singapore Department of Microbiology, Faculty of Medicine, National University of Singapore, Singapore.
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