| Deletion of Tis7 protects mice from high-fat diet-induced weight gain and blunts the intestinal adaptive response postresection. | |
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MedLine Citation:
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PMID: 20861213 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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After loss of intestinal surface area, the remaining bowel undergoes a morphometric and functional adaptive response. Enterocytic expression of the transcriptional coregulator tetradecanoyl phorbol acetate induced sequence 7 (Tis7) is markedly increased in a murine model of intestinal adaptation. Mice overexpressing Tis7 in intestine have greater triglyceride absorption and weight gain when fed a high-fat diet (42% energy) than their wild-type (WT) littermates fed the same diet. These and other data suggest that Tis7 has a unique role in nutrient absorptive and metabolic adaptation. Herein, male Tis7(-/-) and WT mice were fed a high-fat diet (42% energy) for 8 wk. Weight was monitored and metabolic analyses and hepatic and intestinal lipid concentrations were compared after 8 wk. Intestinal lipid absorption and metabolism studies and intestinal resection surgeries were performed in separate groups of Tis7(-/-) and WT mice. At 8 wk, weight gain was less and jejunal mucosal and hepatic triglyceride and cholesterol concentrations were lower in Tis7(-/-) mice than in the WT controls. Following corn oil gavage, serum cholesterol, triglyceride, and FFA concentrations were lower in the Tis7(-/-) mice than in the WT mice. Incorporation of oral (3)[H] triolein into intestinal mucosal cholesterol ester and FFA was less in Tis7(-/-) compared with WT mice. Following resection, crypt cell proliferation rates and villus heights were lower in Tis7(-/-) than in WT mice, indicating a blunted adaptive response. Our results suggest a novel physiologic function for Tis7 in the gut as a global regulator of lipid absorption and metabolism and epithelial cell proliferation. |
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Authors:
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Cong Yu; Shujun Jiang; Jianyun Lu; Carrie C Coughlin; Yuan Wang; Elzbieta A Swietlicki; Lihua Wang; Ilja Vietor; Lukas A Huber; Domagoj Cikes; Trey Coleman; Yan Xie; Clay F Semenkovich; Nicholas O Davidson; Marc S Levin; Deborah C Rubin |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-09-22 |
Journal Detail:
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Title: The Journal of nutrition Volume: 140 ISSN: 1541-6100 ISO Abbreviation: J. Nutr. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-10-21 Completed Date: 2010-11-09 Revised Date: 2013-05-27 |
Medline Journal Info:
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Nlm Unique ID: 0404243 Medline TA: J Nutr Country: United States |
Other Details:
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Languages: eng Pagination: 1907-14 Citation Subset: IM |
Affiliation:
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Division of Gastroenterology, Washington University School of Medicine, St Louis, MO 63110, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adaptation, Physiological
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genetics* Animals Cell Proliferation Dietary Fats / adverse effects* Fatty Liver / genetics, metabolism, pathology Gene Expression Regulation / genetics Immediate-Early Proteins / genetics* Intestinal Absorption / genetics Intestinal Mucosa / metabolism, pathology Intestine, Small / surgery Intestines / metabolism, pathology, physiopathology* Lipid Metabolism / genetics* Lipids / analysis, blood Male Membrane Proteins / genetics* Mice Mice, Inbred C57BL Mice, Knockout RNA, Messenger / metabolism Short Bowel Syndrome / metabolism, pathology, physiopathology* Time Factors Triglycerides / metabolism Weight Gain / genetics* |
| Grant Support | |
ID/Acronym/Agency:
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DK-56260/DK/NIDDK NIH HHS; DK46122/DK/NIDDK NIH HHS; DK50466/DK/NIDDK NIH HHS; DK56341/DK/NIDDK NIH HHS; DK61216/DK/NIDDK NIH HHS; HL-38180/HL/NHLBI NIH HHS; P30 DK52574/DK/NIDDK NIH HHS; R01 DK046122/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Dietary Fats; 0/Ifrd1 protein, mouse; 0/Immediate-Early Proteins; 0/Lipids; 0/Membrane Proteins; 0/RNA, Messenger; 0/Triglycerides |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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