| Deletion of RBP-J in dendritic cells compromises TLR-mediated DC activation accompanied by abnormal cytoskeleton reorganization. | |
| | |
MedLine Citation:
|
PMID: 23138187 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Dendritic cells (DCs) are professional antigen presenting cells that activate and modulate immune responses, but the mechanisms underlying DC activation have not been fully understood. In this study, we investigated the role of Notch signaling in DC activation by using murine bone marrow-derived DCs. Triggering of Toll-like receptors (TLRs) of DCs led to upregulated expression of Notch ligands. Disruption of Notch signaling by the deletion of RBP-J, the critical transcription factor mediating the canonical signaling from all Notch receptors, resulted in a reduced capacity of DCs in activating T cells. Moreover, RBP-J deficiency altered the polarization of T cell activation, as manifested by downregulated interferon-γ and upregulated interleukin-4 and -10 expressions after LPS or Poly(I:C) stimulation. Furthermore, we found that RBP-J(-/-) DCs had reduced intracellular calcium after TLR-triggering. Immunofluorescent staining showed that RBP-J deficient DCs exhibited attenuated cytoskeleton reorganization when contacting T cells. In summary, our results suggested that the canonical Notch signaling promotes the cytoskeleton reorganization and the TLR-mediated DC activation. |
| | |
Authors:
|
Yun-Ru Chen; Fan Feng; Li Wang; Shuo-Yao Qu; Zhen-Qiang Zhang; Li Liu; Hong-Yan Qin; Ying-Min Liang; Hua Han |
Related Documents
:
|
23002437 - Inducible renitence limits listeria monocytogenes escape from vacuoles in macrophages. 23352337 - Metallophilic macrophages of the rodent thymus. 23177537 - Immunity and early pregnancy events: are endocannabinoids the missing link? 23575697 - The interaction between c5a and both c5ar and c5l2 receptors is required for production... 16237197 - Potentiation of apoptosis by heat stress plus pesticide exposure in stress resistant hu... 18487847 - Chronic inflammation and amyloidogenesis in alzheimer's disease -- role of spirochetes. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-11-9 |
Journal Detail:
|
Title: Molecular biology reports Volume: - ISSN: 1573-4978 ISO Abbreviation: Mol. Biol. Rep. Publication Date: 2012 Nov |
Date Detail:
|
Created Date: 2012-11-9 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0403234 Medline TA: Mol Biol Rep Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Department of Hematology, Tangdu Hospital, Fourth Military Medical University, Xi'an, 710038, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Regulation of the major vacuolar Ca(2+) transporter genes, by intercellular Ca(2+) concentration and...
Next Document: Plasma Cyclophilin A Is a Novel Biomarker for Coronary Artery Disease.