| Deletion of Aquaporin 5 Aggravates Acute Lung Injury Induced by Pseudomonas aeruginosa. | |
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MedLine Citation:
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PMID: 21502879 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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BACKGROUND:: Aquaporin (AQP) is a membrane protein that facilitates osmotic water transport. Aquaporin 5 (AQP5) expresses at type I alveolar epithelia of apical membrane that confers high osmotic water permeability. Osmosis or stretch challenge in alveoli significantly up-regulates AQP5 expression, which suggests that AQP5 may play a role in the maintenance of epithelia barrier function. Pseudomonas aeruginosa (PA), a leading gram-negative bacterial frequently isolated from ventilation-associated pneumonia patients, disrupts alveolar and airway epithelial cells and subsequently leads to blood dissemination. In this study, we hypothesized that AQP5 might be protective in acute lung injury induced by PA, and deletion of AQP5 might lead to aggravated lung injury. METHODS:: Lung injury model was induced by intratracheal instillation of PA (1 × 10 colony-forming unit) in wild-type and AQP5 knockout mice, 2 hours and 6 hours later, blood and lung lysate were cultured to detect blood dissemination, bronchoalveolar lavage fluid and lung tissue were collected for histology analysis. Lung injury assessment, wet/dry weight ratio, protein leakage, and Evan's blue dye extravasation were evaluated for pulmonary barrier function. RESULTS:: AQP5 deficiency led to increased bacterial blood dissemination and aggravated lung injury during PA infection, and AQP5 deletion also reduced mucin production in lung. Moreover, AQP5 deficiency showed declined activation of mitogen-activated protein kinase and nuclear factor-kappa B pathways in lungs before and after PA infection. CONCLUSION:: Our data demonstrated that AQP5 plays a protective role in the maintenance of pulmonary barrier function against PA infection. |
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Authors:
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Zi-Qiang Zhang; Yuan-Lin Song; Zhi-Hong Chen; Yao Shen; Chun-Xue Bai |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-4-15 |
Journal Detail:
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Title: The Journal of trauma Volume: - ISSN: 1529-8809 ISO Abbreviation: - Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-4-19 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376373 Medline TA: J Trauma Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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From the Department of pulmonary medicine (Z.-Q.Z., Z.-H.C., Y.S., C.-X.B.), Zhongshan Hospital, Fudan University, Shanghai, People's Republic of China; Department of pulmonary medicine (Z.-Q.Z.), Qianfoshan Hospital of Shandong Province, Shandong University, Jinan, People's Republic of China; and Department of Anesthesia and Perioperative Care (Y.-L.S.), University of California, San Francisco, California. |
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